原创力文档the effect of enzymatically polymerised polyphenols on cd4 binding and cytokine production in murine splenocytes保持酶的聚合多酚的影响在小鼠脾细胞cd4绑定和细胞因子的生产.pdfVIP
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the effect of enzymatically polymerised polyphenols on cd4 binding and cytokine production in murine splenocytes保持酶的聚合多酚的影响在小鼠脾细胞cd4绑定和细胞因子的生产
The Effect of Enzymatically Polymerised Polyphenols on
CD4 Binding and Cytokine Production in Murine
Splenocytes
Daisuke Yamanaka, Yumi Tamiya, Masuro Motoi, Ken-ichi Ishibashi, Noriko N. Miura, Yoshiyuki Adachi,
Naohito Ohno*
Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo, Japan
Abstract
High-molecular weight polymerised polyphenols have been shown to exhibit anti-influenza virus, anti-HIV, and anti-cancer
activities. The purpose of this study was to evaluate the immunomodulating activities of enzymatically polymerised
polyphenols, and to clarify the underlying mechanisms of their effects. The cytokine-inducing activity of the enzymatically
polymerised polyphenols derived from caffeic acid (CA), ferulic acid (FA), and p-coumaric acid (CoA) was investigated using
murine splenocytes. Polymerised polyphenols, but not non-polymerised polyphenols, induced cytokine synthesis in murine
splenocytes. Polymerised polyphenols induced several cytokines in murine splenocytes, with interferon-c (IFN-c) and
granulocyte-macrophage colony-stimulating factor (GM-CSF) being the most prominent. The underlying mechanisms of the
effects of the polymerised polyphenols were then studied using neutralising antibodies and fluorescent-activated cell
sorting (FACS) analysis. Our results show that polymerised polyphenols increased IFN-c and GM-CSF production in
splenocytes. In addition, the anti-CD4 neutralised monoclonal antibody (mAb) inhibited polymerised polyphenol-induced
IFN-c and GM-CSF secretion. Moreover, polymerised polyphenols bound directly to a recombinant CD4 protein, and FACS
analysis confirmed that interaction occurs between polymerised polyphenols and CD4 molecules expres
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