the gut fungus basidiobolus ranarum has a large genome and different copy numbers of putatively functionally redundant elongation factor genes肠道真菌basidiobolus ranarum有大量基因组和不同的副本数量的推定地功能冗余延长因子基因.pdfVIP

The Gut Fungus Basidiobolus ranarum Has a Large Genome and Different Copy Numbers of Putatively Functionally Redundant Elongation Factor Genes Daniel A. Henk*, Matthew C. Fisher The Department of Infectious Disease Epidemiology, Imperial College London, London, United Kingdom Abstract Fungal genomes range in size from 2.3 Mb for the microsporidian Encephalitozoon intestinalis up to 8000 Mb for Entomophaga aulicae, with a mean genome size of 37 Mb. Basidiobolus, a common inhabitant of vertebrate guts, is distantly related to all other fungi, and is unique in possessing both EF-1a and EFL genes. Using DNA sequencing and a quantitative PCR approach, we estimated a haploid genome size for Basidiobolus at 350 Mb. However, based on allelic variation, the nuclear genome is at least diploid, leading us to believe that the final genome size is at least 700 Mb. We also found that EFL was in three times the copy number of its putatively functionally overlapping paralog EF-1a. This suggests that gene or genome duplication may be an important feature of B. ranarum evolution, and also suggests that B. ranarum may have mechanisms in place that favor the preservation of functionally overlapping genes. Citation: Henk DA, Fisher MC (2012) The Gut Fungus Basidiobolus ranarum Has a Large Genome and Different Copy Numbers of Putatively Functionally Redundant Elongation Factor Genes. PLoS ONE 7(2): e31268. doi:10.1371/journal.pone.0031268 Editor: Jason E. Stajich, University of California Riverside, United States of America Received October 6, 2011; Accepted January 5, 2012; Published February 17, 2012 Copyright: 2012 Henk, Fisher. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided