virulence characteristic and mlst-agr genetic background of high-level mupirocin-resistant, mrsa isolates from shanghai and wenzhou, china毒性特点和高层mupirocin-resistant mlst-agr遗传背景,来自中国上海和温州,耐甲氧西林金黄色葡萄球菌分离株.pdfVIP

Virulence Characteristic and MLST-agr Genetic Background of High-Level Mupirocin-Resistant, MRSA Isolates from Shanghai and Wenzhou, China 1 2 2 2 2 Qingzhong Liu , Lizhong Han , Bin Li , Jingyong Sun , Yuxing Ni * 1 Department of Clinical Laboratory, Shanghai First People’s Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China, 2 Department of Clinical Microbiology, Ruijin Hospital, School of Medicine, Shanghai, China Abstract The emergence and prevalence of high-level mupirocin-resistant, methicillin-resistant Staphylococcus aureus (MuH MRSA) is challenging the eradication of MRSA nasal carriage and the treatment of skin and soft tissue infections. To understand the potentially pathogenetic capacity and the genetic basis of MuH MRSA, it is important to have a detailed knowledge of the molecular traits of this organism. Fifty three MuH MRSA isolates were gathered from Shanghai (28 isolates) and Wenzhou (25 isolates) in China. These isolates, consisting of 27 different PFGE-SCCmec-spa patterns, were examined by PCR for 35 virulence genes and further typed using agr (accessory gene regulator) typing and MLST (multilocus sequence typing). All 53 strains were positive for the genes hlg/hlg variant and icaD, and negative for seb, sed, see, seh, eta, etb, hld, cap-5, and ACME-arcA. Compared with Wenzhou isolates, Shanghai isolates were more likely to carry seg (P = 0.002) and several other genes which were not found in Wenzhou strains such as sec, sei, tst (P,0.001 each), and pvl (P = 0.012), and less likely to contain sea (P,0.001), cna (P = 0.031), and efb (P = 0.045). MLST and agr typing showed that ST239-agr1, ST5-agr1, and ST239-agr2 were the common lineages in MuH MRSA isolates from these two different regions. Our results indicated