visualizing escherichia coli sub-cellular structure using sparse deconvolution spatial light interference tomography可视化大肠杆菌亚细胞结构使用稀疏反褶积空间光干涉层析成象.pdfVIP

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visualizing escherichia coli sub-cellular structure using sparse deconvolution spatial light interference tomography可视化大肠杆菌亚细胞结构使用稀疏反褶积空间光干涉层析成象.pdf

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visualizing escherichia coli sub-cellular structure using sparse deconvolution spatial light interference tomography可视化大肠杆菌亚细胞结构使用稀疏反褶积空间光干涉层析成象

Visualizing Escherichia coli Sub-Cellular Structure Using Sparse Deconvolution Spatial Light Interference Tomography 1,2 . 2. 3 1,2 3,4 1,2 Mustafa Mir * , S. Derin Babacan , Michael Bednarz , Minh N. Do , Ido Golding , Gabriel Popescu 1 Department of Electrical and Computer Engineering, University of Illinois at Urbana-Champaign, Urbana, Illinois, United States of America, 2 Beckman Institute for Advanced Science Technology, University of Illinois at Urbana-Champaign, Urbana, Illinois, United States of America, 3 Department of Physics, Center for the Physics of Living Cells, University of Illinois at Urbana-Champaign, Urbana, Illinois, United States of America, 4 Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas, United States of America Abstract Studying the 3D sub-cellular structure of living cells is essential to our understanding of biological function. However, tomographic imaging of live cells is challenging mainly because they are transparent, i.e., weakly scattering structures. Therefore, this type of imaging has been implemented largely using fluorescence techniques. While confocal fluorescence imaging is a common approach to achieve sectioning, it requires fluorescence probes that are often harmful to the living specimen. On the other hand, by using the intrinsic contrast of the structures it is possible to study living cells in a non- invasive manner. One method that provides high-resolution quantitative information about nanoscale structures is a broadband interferometric technique known as Spatial Light Interference Microscopy (SLIM). In addition to r