water-soluble electrospun nanofibers as a method for on-chip reagent storage水溶性静电纺丝作为片上试剂存储方法.pdfVIP

Biosensors 2012, 2, 388-395; doi:10.3390/bios2040388 OPEN ACCESS biosensors ISSN 2079-6374 /journal/biosensors/ Communication Water-Soluble Electrospun Nanofibers as a Method for On-Chip Reagent Storage Minhui Dai 1, Shengquan Jin 1,2 and Sam R. Nugen 1,* 1 University of Massachusetts, 102 Holdsworth Way, Amherst, MA 01003, USA; E-Mails: mdai@ (M.D.); haiquanjsq@ (S.J.) 2 Lab of Biosystems and Microanalysis, State Key Laboratory of Bioreactor Engineering, East China University of Science Technology, Shanghai 200237, China * Author to whom correspondence should be addressed; E-Mail: snugen@; Tel.: +1-413-545-1025; Fax: +1-413-545-1262. Received: 15 August 2012; in revised form: 15 September 2012 / Accepted: 28 September 2012 / Published: 8 October 2012 Abstract: This work demonstrates the ability to electrospin reagents into water-soluble nanofibers resulting in a stable on-chip enzyme storage format. Polyvinylpyrrolidone (PVP) nanofibers were spun with incorporation of the enzyme horseradish peroxidase (HRP). Scanning electron microscopy (SEM) of the spun nanofibers was used to confirm the non-woven structure which had an average diameter of 155  34 nm. The HRP containing fibers were tested for their change in activity following electrospinning and during storage. A colorimetric assay was used to characterize the activity of HRP by reaction with the nanofiber mats in a microtiter plate and monitoring the chan