转录产物MALA1高表达：及早期非小细胞型肺癌转移有关.docVIP

转录产物MALA1高表达：及早期非小细胞型肺癌转移有关 　 作者：王文兵，周密，严丽荣， 乌慧玲 【摘要】 　　目的： 研究转录产物MALA1的表达水平与早期非小细胞型肺癌(NSCLC)转移之间的关系。方法： 用实时定量PCR分析了MALA1基因在70例NSCLC患者中的表达情况。 结果： 在对Ⅰ期和Ⅱ期NSCLC患者超过5年的随访期中发现，MALA1高表达的患者死亡率超过40 %(12/28)，而MALA1低表达的患者死亡率仅有9 %(2/22) （P=0.01)。说明高表达量的MALA1很可能是早期NSCLC 患者预后不良的一个指标。此外，也发现了MALA1基因与肿瘤组织的转移有关。结论： MALA1的转录水平不仅可以作为预测发生肿瘤转移的高危人群的生物学标记，并有望在今后用于早期NSCLC患者的诊断或治疗中。 【关键词】 转移； 非小细胞型肺癌； MALA1； 表达； 扣除杂交 　　［Abstract］Objective: To investigate the relationship of the expression level of the transcript MALA1 and the metastasis of early stage nonsmall cell lung cancer(NSCLC).Methods： Expression level of MALA1 was analyzed with real time quantitative PCR in 70 patients of NSCLC.Results： In the study of an over fiveyear followup for stage Ⅰand Ⅱ NSCLC patients.It was found that the death rate was more than 40% in patients with high MALA1 expression, whereas only 9%(2/22) in patients with low MALA1 expression died(P=0.01), suggesting high expression of MALA1 was potentially predictive for a poor prognosis in early NSCLC.Additionally, it was found that the association of MALA1 gene with metastasis depended on the tumor′s histology.Conclusion： These results demonstrated that MALA1 transcript could be used as a biological marker, which may predict high risk for the development of distant metastasis and could be further helpful in improvement of treatment for earlystage NSCLC patients. 　　［Key words］ metastasis; nonsmall cell lung carcinoma; MALA1; expression; subtractive hybridization Bronchogenic carcinoma continues to be the leading cause of cancer death in both men and women ［1］.Nonsmall cell lung carcinoma(NSCLC) accounts for approximately 80% of all bronchogenic carcinomas, with SCLC(small cell lung carcinoma) accounting for the remainder［1］.Survival following treatment of NSCLC is stagerelated, and the patients with earlystage disease have the best chance for curative treatment.Although the patients with stage Ⅰ and Ⅱ disease undergo surgical resection of