转录因子PAX5在儿童急性白血病细胞中表达.docVIP

转录因子PAX5在儿童急性白血病细胞中表达 　 作者：张蓓，帖利军，叶启东，顾龙君，汤静燕，袁向亮，沈立松 【摘要】 为了观察了解儿童急性白血病细胞中转录因子PAX5的表达特性，采用实时定量RT-PCR方法测定了6个血液肿瘤细胞株以及6例正常儿童、58例初发和4例复发急性白血病儿童（其中包括39例B-ALL，10例T-ALL和13例AML）骨髓细胞中PAX5和CD19 mRNA的表达水平。结果表明：在Namalwa（B细胞系）细胞株中，PAX5和CD19 mRNA相对表达量分别为2.35%和2.52%；而在T-和髓系细胞株中几乎不表达。在临床样本中B-ALL组比T-ALL组和AML组的PAX5 mRNA表达高（P=0.029和P=0.013）。T-ALL组和AML组的PAX5 mRNA表达水平均低于正常对照组，而T-ALL组与AML组之间的表达差异无显著意义。在B-ALL患儿中，PAX5 mRNA表达的个体差异很大。此外还发现，初发治疗前组和复发组的B-ALL患儿PAX5 mRNA表达高于化疗完全缓解组（P=0.011和P=0.006）。由于在CD19基因的启动子上有B细胞特异性激活蛋白的结合位点，研究发现在B-ALL中PAX5表达水平与同样用实时定量RT-PCR检测的CD19表达水平之间存在明显的相关性。结论：运用实时定量RT-PCR方法能快速准确地在B-ALL的临床标本中检测和定量PAX5基因的表达。研究中发现部分B-ALL中PAX5 mRNA表达明显升高，因此它可将其作为进一步研究B-ALL发病机理的另一个切入点。 【关键词】 转录因子 　　Expression of the Transcription Factor PAX5 in Childhood Acute Leukemic Cells 　Abstract To investigate transcription factor PAX5 expression characteristics in childhood acute leukemic cells，expression levels of PAX5 and CD19 mRNA in 6 hematological tumor cell lines and bone marrow cells of 6 normal children，58 de novo patients and 4 relapse acute leukemic children，including 39 cases of B-ALL，10 cases of T-ALL and 13 cases of AML，were detected by a real-time RT-PCR. The results showed that PAX5 and CD19 mRNA expression levels were 2.35% and 2.52% in Namalwa (B-cell lines) respectively，but almost not detectable in other T- and myeloid cell lines. Among clinical samples，expression of PAX5 mRNA in B-ALL was significantly higher than that in T-ALL and AML(P=0.029 and P=0.013 respectively). PAX5 expression was significantly lower in T-ALL and AML than that in normal controls.The difference of PAX5 mRNA expression levels between T-ALL and AML was not significant. Individual difference of PAX5 mRNA expression levels in children with B-ALL was great. Moreover，PAX5 mRNA expressions in de novo and relapse patients with B-ALL were significantly higher than those in remission (P=0011 and P=0.006 respectively). As binding sites for B-cell specific activator protein have been identified in the promoter regi