逐淤化痰汤对脑出血大鼠细胞凋亡及Bcl-2、Bax蛋白表达影响.docVIP

逐淤化痰汤对脑出血大鼠细胞凋亡及Bcl-2、Bax蛋白表达影响 　【摘要】 目的 探讨逐淤化痰汤对脑出血大鼠血肿周围脑组织细胞凋亡及Bcl-2、Bax蛋白表达的影响。方法 90只SD大鼠随机分为逐淤化痰汤组、脑出血组和假手术组，每组30只。用尾状核注射自体血法建立脑出血模型；在不同时间点以Hoechst法检测神经细胞凋亡数及免疫组化法检测Bcl-2、Bax蛋白表达的变化。结果 出血组脑组织细胞凋亡数和Bcl-2、Bax蛋白表达水平均明显高于假手术组（P＜0.01）；与出血组相比，逐淤化痰汤组细胞凋亡数与Bax蛋白表达水平均下降（P＜0.05或P＜0.01），而Bcl-2蛋白表达水平显著增高（P＜0.05或P＜0.01）。结论 逐淤化痰汤可抑制出血后神经细胞凋亡，此作用可能与其上调Bcl-2和下调Bax蛋白表达水平有关。 【关键词】 逐淤化痰汤 脑出血 细胞凋亡 Bcl-2蛋白 Bax蛋白 　　Abstract: Objective To probe into the effect of edaravone on the cell apoptosis and the expression of Bcl-2 and Bax protein following intracerebral hemorrhage(ICH) in rats, and explore the protective effect of edaravone on the cerebral injury induced by hemorrhage. Methods Ninety SD rats were randomly divided into 3 groups: Zhuyuhuatan oral liquid group, ICH group and sham group(n=30).The rat model of ICH was established by injection of autologous blood into the caudate nucleus. At different time separately neuronal apoptosis and the expression of Bcl-2 and Bax protein were monitored by Hoechst and immunohistochemistry,respectively. Results Apoptosis and the expression of Bcl-2 and Bax protein were significantly increased in the ICH group as compared with the sham group(P＜0.01). Treatment of Zhuyuhuatan oral liquid markedly reduced apoptosis ahd the expression of Bax protein in comparison to the ICH group(P＜0.05 or ＜0.01),while the expression of Bcl-2 was obviously higher in the Zhuyuhuatan oral liquid group than that in the ICH group(P＜0.05 or P＜0.01). Conclusions Zhuyuhuatan oral liquid inhibits the neuronal apoptosis following intracerebral hemorrhage which might be relative to the alleviated oxidative stress, up regulated Bcl-2 protein and down regulated expression of Bax protein. Key words:Zhuyuhuatan oral liquid; cerebral hemorrhage; apoptosis; Bcl-2 protein; Bax protein 脑出血（intracerebral hemorrhage,ICH）是神经系统常见病和多发病。研究表明，细胞凋亡机制参与了脑出血继发性神经细胞损伤［1，2］。现阶段中药方剂在脑出血的治疗中仍然发挥着重要作用，但传统观念认为有引起出血加重危险，故多应用于脑出血恢复期。最新研究表明，脑出血急性期应用逐淤化痰药物疗效更好［3］。逐淤化痰汤（Zhuyuhuatan or