镁对大鼠离体缺血再灌注心肌作用.docVIP

镁对大鼠离体缺血再灌注心肌作用 　 作者：张君平，杜友爱，郭益民，刘重斌，余立群 【摘要】 目的:观察在缺血再灌注5 min后应用镁剂是否能对离体缺血再灌注心肌起到保护作用。方法:建立Langendorff离体心脏缺血再灌注模型，结扎冠状动脉左前降支，缺血20 min，复灌60 min，高镁组在复灌5 min时应用浓度为2.4 mmol/L、4.8 mmol/L、8.4 mmol/L的高镁K-H液灌注10 min。观察心脏血流动力学指标，实验结束时测心肌匀浆MDA含量，免疫组化检测SOD的含量。结果:①复灌15 min时三个高镁组LVSP、LVDP×HR、LV±dp/dtmax明显低于缺血再灌组。②复灌60 min时三个高镁组各指标与缺血再灌组相比差异无显著性。③三个高镁组MDA含量明显低于缺血再灌组，SOD阳性表达明显高于缺血再灌组。结论:缺血再灌注5 min应用高镁K-H液对缺血再灌注心肌的即时作用表现为抑制心脏的血流动力学，复灌结束测得的MDA含量说明镁对心肌有保护作用，体现为抗脂质氧化作用。 【关键词】 高镁；心肌；缺血再灌注 Abstract: Objective: To investigate the role of high magnesium on rat isolated hearts after 5 min of ischemia/reperfusion. Methods: Cardiac hemodynamics indicators were analyzed with the Langendorff method in rat’s isolated heart. MDA was further determined in the experiment. All isolated hearts were subjected to 20 min of ischemia followed by 60 min of reperfusion. Results: ①The groups of high magnesium, perfused with 2.4 mmol/L, 4.8 mmol/L, 8.4 mmol/L magnesium after 5 min of reperfusion for 10 min, left ventricular systolic pressure, multiplication of left ventricular developed pressure and heart rate, and left ventricular±dp/dtmax were predomintly decreased compared with control group (Plt;0.05). ②All indicators in the end of reperfusion had no statistical difference compared with the control group. ③The MDA concentration of the high magnesium groups was significantly lower than that of the control group, SOD of the high magnesium groups was significantly higher than that of the control group (Plt;0.05). Conclusion: Increased magnesium can inhibit myocardium, while it can protect heart from ischemia/reperfusion injury. The cardioprotection may be through anti-lipid-oxidation to decrease MDA concentration. Key words: high magnesium; myocardium; ischemia/reperfusion 心肌缺血再灌注损伤临床主要表现为再灌注性心律失常、心功能下降和心肌坏死面积扩大等。近年来，镁在心血管疾病中的作用日益受到重视，在体和离体缺血再灌注研究[1-4]说明，在缺血期或再灌注即时应用高镁对心肌缺血再灌注损伤具有保护作用。本实验观察在缺血再灌注5 min后应用镁剂对缺血再灌心肌的作用。 1 材料和方法 1.1 实验动物和试剂 Sprague-Dawley 雄性大鼠（体重200～250 g）40只，由温州医学院实验动物中心提供。MDA试剂盒购自南京建