CD4+CD25+调节性T细胞在肝移植术后腹腔感染中表达及其作用.doc

CD4+CD25+调节性T细胞在肝移植术后腹腔感染中表达及其作用 　【摘要】 目的 研究移植后细菌感染CD4+CD25+调节性T细胞(Treg)表达的变化。方法 以DA大鼠为供体，LEW大鼠为受体，采用改良的Kamada二袖套法建立肝移植模型。术后随机分为3组，G1组术后3 d腹腔生理盐水注射；G2组术后3 d给予腹腔大肠杆菌注射；G3组术后常规给予免疫抑制药物CsA，3 mg/(kg·d)。感染后7 d处死动物，每组6个样本，检测血清ALT、TB，并采用流式细胞术检测脾脏Treg的水平，RTPCR方法检测脾脏Foxp3和肝脏IL10、TGFβ mRNA的表达。结果 G3、G2组Treg表达水平显著升高，分别为：(16.72±0.95)%，(27.4±1.09)%，与G1(9.88±0.98)%比较差异均有统计学意义(P＜0.01)；脾脏Foxp3和肝脏IL10、TGFβ mRNA表达水平均增加；感染组的肝功虽然较差，与排斥组相比无统计学意义，但从病理切片观察该组肝脏的急性免疫排斥反应减轻，Banff评分有统计学意义。结论 大鼠肝移植术后腹腔细菌感染增加了脾脏Treg的表达，促进了免疫抑制作用的发挥，部分减轻了急性排斥反应。 【关键词】 肝移植； 腹腔感染； CD4+CD25+调节性T细胞； 急性排斥反应 【Abstract】 Objective To investigate expression of CD4+CD25+ regulatory T cells in model of abdominal cavity bacterial infection after liver transplantation in rats. Methods Orthotopic liver transplant (OLT, DA to Lewis) models were established by modified Kamada twocuff technique. All rats were randomly divided into 3 groups after operation. Normal saline was injected into abdominal cavity at 3rd day postoperatively in group 1, mixed Bacillus coli liquid in group 2 and CsA at 3 mg/kg per day in group 3. Six recipients were sacrificed 7 days after infection in each group, blood and graft samples were collected for detection of ALT and TB. The levels of Treg and Foxp3 in spleens and IL10, TGFβ mRNA in livers were detected by flow cytometry and RTPCR, respectively. Results Compared with the level of Treg for 9.88±0.98 in group 1, the levels of Treg increased obviously in groups 2 and 3 (16.72±0.95 vs 27.4±1.09), with statistical difference (Plt;0.01), so do the expressions of Foxp3, IL10 and TGFβ mRNA. Although the serum concentrations of AST and TB 7 d in Group 2 was higher than those in Group 1, the Banff scores of grafts in group 2 were lower than those in group 1 (Plt;0.05). Conclusions Abdominal cavity bacterial infection after liver transplantation in rats increase expression of Treg, promote the immunosuppressive action and partially alleviate the acute rejection. 【Key words】 Liver transplantation