Ghrelin对重症急性胰腺炎大鼠胃肠运动影响及机制探究.docVIP

Ghrelin对重症急性胰腺炎大鼠胃肠运动影响及机制探究 　【摘要】 目的 观察Ghrelin对重症急性胰腺炎大鼠胃肠运动的影响，同时探讨其机制。方法 Wistar大鼠随机分为对照组、模型组、Ghrelin治疗组，测量肠转运系数；全细胞膜片钳技术记录各组大鼠胃窦环形平滑肌细胞钙敏感性钾电流的数值变化。结果 模型组大鼠肠转运系数明显低于对照组（Plt;0.05），钙敏感性钾电流高于对照组（Plt;0.01）；治疗组转运系数高于模型组（Plt;0.05），而电流明显降低（P=0.01）。结论 重症急性胰腺炎大鼠的胃肠运动减弱，胃肠道平滑肌细胞膜钙敏感性钾电流增加是一个重要的电生理机制，Ghrelin可以通过降低该电流而促进其胃肠运动。 【关键词】 Ghrelin 重症急性胰腺炎 胃肠运动 膜片钳 钙敏感性钾电流 Influence of Ghrelin on gastrointestinal motility in severe acute pancreatitis rats and research on mechanism ［Abstract］ Objective To observe the influence of Ghrelin on gastrointestinal motility in severe acute pancreatitis (SAP) rats，and study on its mechanism.Methods Wistar rats were randomly divided into control group，SAP model group and Ghrelin treatment group.The changes of their intestinal transit index were measured;The whole cell patch clamp technique was used to record the calcium activated potassium current of their gastric antral circular smooth muscle cells.Results The intestinal transit index of model group was lower than that of control group（P＜0.05），and its calcium activated potassium current was higher than control group（P＜0.01）;The index of Ghrelin treatment group was higher than that of control group（P＜0.05），and its current was lower than control group（P=0.01）.Conclusion The gastrointestinal motility of severe acute pancreatitis rat was attenuated，the mechanism of which might be the calcium activated potassium current of its gastrointestinal smooth muscle cell was increased.Ghrelin can promote the gastrointestinal motility of severe acute pancreatitis rat with cutting down the currents. ［Key words］ ghrelin;severe acute pancreatitis;gastrointestinal motility;patch clamp;calcium activated potassium current 重症急性胰腺炎（severe acute pancreatitis，SAP）是一种发病急、并发症多且死亡率高的疾病，有效地控制后期感染和内毒素血症的发生是治疗SAP的关键。通过促进胃肠蠕动排泄毒素，可以达到调整肠道微生态平衡，减少内毒素吸收的作用，Ghrelin是生长激素促分泌剂（GHSs）受体的内源性配体，在胃肠道表现出和胃动素类似的促进胃肠运动作用，本研究以胆盐诱导的重症急性胰腺炎大鼠为模型探讨Ghrelin对其胃肠运动的影响，并且通过观察胃窦平滑肌细胞膜钙敏感性钾电流［calcium activated potassium currents，IK（Ca）］的变化进一步对