儿童急性白血病MDM2基因表达.docVIP

儿童急性白血病MDM2基因表达 　 作者：刘壮，谭丽珍，吴香芩，张义堂，韦红英 【摘要】 目的 探讨MDM2基因mRNA的表达与儿童急性白血病（AL）的关系。方法 采用逆转录多聚酶链式反应技术（RT-PCR），对42例初发或复发AL患儿和13例完全缓解AL(AL-CR)患儿及15例非恶性疾病患儿骨髓单个核细胞进行MDM2 mRNA表达的检测。结果 AL组中MDM2 mRNA的表达率为57.1%，明显高于AL-CR组和对照组；完全缓解组和对照组差异无显著性。MDM2 mRNA的表达与白细胞总数及临床危险度分型有关，与性别、年龄、免疫分型无明显相关性。MDM2 mRNA表达者缓解率低。结论 儿童AL中存在MDM2 mRNA过表达，且可能与儿童AL发病有关；MDM2 mRNA表达是AL的不良预后因素；AL MDM2 mRNA表达者可以降低AL的化疗敏感性，MDM2 mRNA表达对AL有疗效判断和预后的价值。 【关键词】 急性白血病；儿童； MDM2； 逆转录多聚酶链式反应 Expression of MDM2 gene in childhood acute leukemia LIU Zhuang, TAN Li-zhen, WU Xiang-qin, et al.Department of Pediatrics, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021,China 【Abstract】 Objective To investigate the expression and significance of MDM2 gene in childhood acute leukemia (AL). Methods The mRNA expression of MDM2 gene was examined by RT-PCR in bone marrow cells from 42 cases of incipient or relapsed childhood AL, 13 cases of complete remission AL (AL-CR) and 15 children without malignance disease as control group.Results 57.1% AL cases were positive for MDM2 mRNA, remarkably significant higher than that of control group and AL-CR group. There was no difference of MDM2 mRNA expression between AL-CR group and control group. The expression of MDM2 mRNA was connected with initial white blood cell (WBC) count and clinical risk type of AL. There was no connection between MDM2 mRNA expression and some clinical and laboratory parameters such as age, sex and immunogenic type. The remission rate in patients with high expression of MDM2 mRNA was lower. Conclusion There is over expression of MDM2 mRNA in childhood acute leukemia, which may be related to pathogenesis of AL. The expression of MDM2 may imply higher risk and lower sensitivity of the leukemic cells to chemotherapy. The expression of MDM2 mRNA might be the index of treatment effect and poor prognosis. 【Key words】 acute leukemia; child; MDM2; reverse transcription-polymerase chain reaction MDM2(murine double minute 2)基因是从自然转化的Bal B/C小鼠细胞株中发现