大肠癌围手术期血清ｓＦａｓ／ｓＦａｓＬ水平检测临床意义.docVIP

大肠癌围手术期血清ｓＦａｓ／ｓＦａｓＬ水平检测临床意义 　　　【摘要】 目的 探讨大肠癌患者围手术期sFas/sFasL的变化及其临床意义。方法 采用双抗体夹心酶联免疫法(ELISA)测定56例大肠癌患者手术前后血清sFas/sFasL的变化，并与健康对照组比较分析。结果 大肠癌组治疗前sFas/sFasL均增高，与对照组比较有显著性差异(Plt;0．01)；且与淋巴结转移和肿瘤临床Dukes分期有明显相关性(Plt;0．05)。行根治性手术组术后sFas/sFasL均有显著性降低(Plt;0．05),而姑息性手术组sFas/sFasL则无显著性降低(Pgt;0．05)。结论 大肠癌患者血清sFas/sFasL的水平与肿瘤的浸润转移和病程有关，检测大肠癌患者血清sFas/sFasL的变化，有助于估计患者的预后。 　　【关键词】大肠癌；可溶性Fas；可溶性Fas配体；手术 　　 　　【Abstract】 Objective To investigate the changes of serum sFas/sFasL in patients with colorectal cancerbefor and after operation,and evaluate their clinical significance.Methods The contents of serum sFas/sFasL in 56 patients with colorectal cancer were detectedbyELISArespectivelybefore and after oprerationand compared them with that of health controls.Results The contents of serum sFas/sFasLin the patients with colorectal cancerbefore operation were significantly higher than that in control group(Plt;0．01).There was significant correlation between the contents with lymph node metastasis and clinical stage(Dukes)(Plt;0．05).The contents of sFas/sFasL were significantly decreased in patients with colorectal cancer after radical operation(Plt;0．05).But there was not significant decrease of the contents of sFas/sFasL in patients with colorectal cancer after palliative resection(Pgt;0．05).Conclusion The contents of serumsFas/sFasLare closely related to tumor invasion,metastasis and clinical stage(Dukes).Dynamic determination of the contents of serum sFas/sFasL may be an important index for evalution of invasion,metatasis,efficiacy and progrosis for the patients with colorectal cancer 　　【Key words】Colorectal cancer;sFas；sFasL;Operation 　　 　　大肠癌是严重危害人类健康的常见恶性肿瘤之一，由于其发病隐匿，临床诊断时常常失去了根治性手术治疗的机会。研究表明细胞凋亡在大肠癌的发生、发展上起重要的作用[1]。Fas/FasL系统是近年来受到普遍重视的介导细胞凋亡的信号传导系统，属TNF家族成员。当Fas与FasL结合，可向细胞传递死亡信号，而导致细胞凋亡快速发生[2]。Fas(Apo-1或CD95)主要以膜受体形式存在，还可通过mRNA水平的不同剪接方式而产生缺乏跨膜区的可溶性Fas分子(sFas)。sFas可与胞膜的Fas竞争结合可溶性Fas配体(sFasL)而对细胞凋亡起调节作用。因此，探讨大肠癌患者手术前后血清可溶性Fas/FasL变化对了解患者病情发展和指导治疗可能具有一定意义。笔者检测56例大肠癌患者围手术