大鼠孤束核在电针保护应激性胃黏膜损伤中作用.docVIP

大鼠孤束核在电针保护应激性胃黏膜损伤中作用 　【摘要】 目的 探讨大鼠孤束核（NTS）在电针(EA)保护应激性胃黏膜损伤中的作用。方法 将56只健康雄性SD大白鼠随机分为应激组、EA+应激组、NTS电损毁组、NTS假损毁组。通过脑立体定向仪电损毁大鼠孤束核，采用束缚浸水制备大鼠应激性胃溃疡模型，分别检测各组大鼠胃溃疡指数（UI）、胃黏膜血流量（GMBF）、胃液酸度。结果 EA+应激组UI明显减少，GMBF增加，胃酸分泌量减少。与应激组比较有显著性差异（Plt;0.01，Plt;0.001）。NTS电损毁组UI明显提高，GMBF减少，胃酸分泌量增加。分别与NTS假损毁组和EA+应激组比较，差异均有显著性（Plt;0.05或Plt;0.001）。结论 孤束核参与了电针足三里穴抗大鼠应激性胃黏膜损伤作用过程。 【关键词】 孤束核；电损毁；电针；应激性胃溃疡。 ABSTRACT:Objective To explore the role of nucleus tractus solitarious (NTS)in protective effect of electroacupuncture(EA) against stress gastric mucosal lesion in rats. Methods 56 SD male rats were randomly divided into the stress group,EA+stress group,NTS electricity damaged group and sham damaged group.The NTS damaged in the Brain Stereotaxic Frame. The gastric stress ulcer model was established by restrain and water immersion. The ulcer index (UI), gastric mucosa blood flow (GMBF) and acidity of gastric juice were measured. Results The EA+stress group showed that the UI and acidity of gastric juice were significantly reduced and the GMBF was significantly increased, there were obviously difference as compared with the stress group(Plt;0.01，Plt;0.001). The NTS electricity damaged group showed that the UI and acidity of gastric juice were significantly increased and the GMBF was significantly decreased, there were obviously difference as compared with the sham damaged groups or EA+stress group(Plt;0.05 or Plt;0.001).Conclusion NTS is involved in the process of electroacupuncture against stress gastric mucosal lesion. KEY WORDS: Nucleus tractus solitarious；Electricity damage；Electroacupuncture；Gastric stress ulcer 针刺足三里穴可调节胃的功能，不仅可治疗而且可以预防胃黏膜损伤，促进溃疡的愈合[1，2]，但其确切的机制目前尚未完全明了。延髓内脏带内部以孤束核（nucleus tratus solitarius,NTS）为中心，通过诸多核团之间存在的纤维联系，构成以延髓内脏活动调控为主的中枢，参与躯体性、内脏性感觉和内脏活动的调节，是一重要的应激反应调控中枢[3，4]，但NTS是否参与电针抗大鼠应激性胃溃疡作用尚未见报道。本实验在前期研究证明电针足三里穴对应激胃黏膜损伤保护作用的基础上，进一步观察电损毁NTS后，电针足三里穴对大鼠应激性胃溃疡的影响，以探讨NTS在电针保护胃黏膜损伤中的作用。 1 材料和方法 1.1 实验动物及分组 健康成年雄性SD大鼠，共56只，体重230±20g，由本院实验动物中心提供。动物随机分为4组：①应激组；②EA+应激组；③NT