the outcome of phagocytic cell division with infectious cargo depends on single phagosome formation吞噬细胞分裂与传染性货物的结果取决于单一吞噬体的形成.pdfVIP

The Outcome of Phagocytic Cell Division with Infectious Cargo Depends on Single Phagosome Formation 1 1 2 1,3 Yong Luo *, Mauricio Alvarez , Lingchuan Xia , Arturo Casadevall 1 Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York, United States of America, 2 Jiaomen 13th Yuan, Beijing, People’s Republic of China, 3 Department of Medicine, Albert Einstein College of Medicine, Bronx, New York, United States of America Abstract Given that macrophages can proliferate and that certain microbes survive inside phagocytic cells, the question arises as to the post-mitotic distribution of microbial cargo. Using macrophage-like cells we evaluated the post-mitotic distribution of intracellular Cryptococcus yeasts and polystyrene beads by comparing experimental data to a stochastic model. For beads, the post-mitotic distribution was that expected from chance alone. However, for yeast cells the post-mitotic distribution was unequal, implying preferential sorting to one daughter cell. This mechanism for unequal distribution was phagosomal fusion, which effectively reduced the intracellular particle number. Hence, post-mitotic intracellular particle distribution is stochastic, unless microbial and/or host factors promote unequal distribution into daughter cells. In our system unequal cargo distribution appeared to benefit the microbe by promoting host cell exocytosis. Post-mitotic infectious cargo distribution is a new parameter to consider in the study of intracellular pathogens since it could potentially define the outcome of phagocytic-microbial interactions. Citation: Luo Y, Alvarez M, Xia L, Casadevall A (2008) The Outcome of Phagocytic Cell Division with Infectious Cargo Depends on Single Phago