the origin recognition complex protein family原点识别复杂的蛋白质家族.pdfVIP

Protein family review TThhee oorriiggiinn rreeccooggnniittiioonn ccoommpplleexx pprrootteeiinn ffaammiillyy Bernard P Duncker*, Igor N Chesnokov† and Brendan J McConkey* Addresses: *Department of Biology, University of Waterloo, Waterloo, Ontario, N2L 3G1 Canada. †Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, School of Medicine, Birmingham, AL 35294, USA. Correspondence: Bernard P Duncker. Email: bduncker@sciborg.uwaterloo.ca Published: 17 March 2009 Genome BBiioollooggyy 2009, 1100::214 (doi:10.1186/gb-2009-10-3-214) The electronic version of this article is the complete one and can be found online at /2009/10/3/214 © 2009 BioMed Central Ltd SSuummmmaarryy Origin recognition complex (ORC) proteins were first discovered as a six-subunit assemblage in budding yeast that promotes the initiation of DNA replication. Orc1-5 appear to be present in all eukaryotes, and include both AAA+ and winged-helix motifs. A sixth protein, Orc6, shows no structural similarity to the other ORC proteins, and is poorly conserved between budding yeast and most other eukaryotic species. The replication factor Cdc6 has extensive sequence similarity with Orc1 and phylogenetic analysis suggests the genes that encode them may be paralogs. ORC proteins have also been found in the archaea, and the bacterial DnaA replication protein has ORC-like functional domains. In budding yeast, Orc1-6 are bound to origins of DNA replication throughout the cell cycle. Following association with Cdc6 in G1 phase, the sequential hydrolysis of Cdc6- then ORC-bound ATP loads the Mcm2-7 helicase complex onto DNA. Localization of ORC subunits to the kinetochore and centrosome during mitosis an