第十一章植物组织培养（The eleventh chapter is plant tissue culture）.doc

第十一章植物组织培养（The eleventh chapter is plant tissue culture） The eleventh chapter is plant tissue and cell culture Some concept It is based on the plant tissue cell is the basic unit of cultivation, breeding or artificial manipulation in vitro, the biological characteristics of cells according to the will of the people change, so as to create new or improved varieties or species, accelerate the reproduction of individual plants, or to obtain valuable substances are called plant cell engineering. Plant cell engineering The main contents of it in plant cell engineering Tissue culture and plant cell and organ \ \ The technology of Anther Culture The plant protoplast preparation The fusion of plant protoplasts The protoplasm uptake experiment The production of transgenic plants The chromosome (Group) operation technology This chapter introduces the culture of plant cells, tissues and organs Two, the history of plant cell engineering In 1902, the German botanist Haber Lent (G.Haberlandt), the earliest plant cell culture experiment, known as the father of plant tissue culture; In 1904, Hanning cultured radish and horseradish embryos in inorganic salt and sucrose solution. The technique of immature embryo culture was firstly applied to plant cell engineering in breeding practice. In 1922, the cultures of root tips were reported in the United States and germany; From 1934 to 1939, many kinds of auxin have been found, and in vitro culture of tomato and carrot have been used to establish a more mature method of cell culture, which makes plant cell culture enter a new era; 1941. Holland J. Van Overbeek demonstrated that active components of coconut milk promote the division and growth of other types of cells in vitro. In 1943, White (White) proposed that plant cells are totipotent 1948. Chinese plant physiologist Cui Zhi has worked with American scientists to find that the ratio of adenine to auxin is one of the major factors controlling bud formation and root formation. 1954.skoo