氧化亚铁硫杆菌中磁小体形成相关基因在不同亚铁浓度刺激下的差异.PDF

生 物 工 程 学 报 Chin J Biotech 2009, January 25; 25(1): 69-75 Chinese Journal of Biotechnology ISSN 1000-3061 cjb@ © 2009 Institute of Microbiology, CAS CSM, All rights reserved 环境生物技术 刘新星, 武海艳, 刘文斌, 李寿朋 , 410083 : 氧化亚铁硫杆菌(Acidithiobacillus ferrooxidans ) 的生物控制矿化作用可以使其在胞内形成黑色电子致密颗粒— 磁小体。本研究利用生物信息学方法对氧化亚铁硫杆菌标准菌株 ATCC 23270 的全基因组进行分析, 并通过 Real-time PCR 技术研究氧化亚铁硫杆菌中与磁小体形成相关的mpsA 、magA 、thy 和mamB 四个基因在不同亚铁浓度刺激下的差 异表达, 结果发现它们在转录层面的表达量受亚铁浓度的影响, 当亚铁浓度达到150~200 mmol/L 范围内达到最高表达, 这对进一步深入研究氧化亚铁硫杆菌中磁小体的形成机理有积极的意义。 : 氧化亚铁硫杆菌, 磁小体, 生物信息学, 实时定量PCR Response of genes for synthesizing the magnetic of Acidithiobacillus ferrooxidans to different concentration of Fe2+ stress Xinxing Liu, Haiyan Wu, Wenbin Liu, and Shoupeng Li Department of Bioengineering , School of Resources Processing and Bioengineering, Central South University, Changsha 410083, China Abstract: Acidithiobacillus ferrooxidans is able to synthesize intra-cellular electron-dense magnetite, which formed by BCM method in Acidithiobacillus ferrooxidans . The whole genome of the type strain Acidithiobacillus ferrooxidans ATCC 23270 was analyzed by bioinformatics and some homolog genes of functional ones in magnetotactic bacteria were available. This study analyzed the different concentration of Fe2+ stress response of mpsA, magA, thy and mamB gene by using real-time PCR analysis. Temporal genes expression profiles were examined in cells subjected to different concentration of FeSO ·7H O stress, they reached to high expression