人脑星形细胞瘤组织中环氧化酶2表达及其临床意义.docVIP

人脑星形细胞瘤组织中环氧化酶2表达及其临床意义 　 作者：徐滨，宋来君，刘献志，徐国本，张智峰 【关键词】 星形细胞瘤；前列腺素内过氧化物合酶；逆转绿聚合酶链反应；免疫组织化学 　　Expression and clinical significance of cyclooxygenase2 in human astrocytoma 　　【Abstract】 AIM: To investigate the role of cyclooxygenase (COX) in the development and progression of human astrocytomas through examining the expressions of COX1 and COX2 in human astrocytoma tissue and normal brain tissue. METHODS: Reverse transcriptasepolymerase chain reaction(RTPCR) and immunohistochemistry(SP) were applied to detect the mRNA and protein expressions of COX1 and COX2 in human astrocytoma tissue and normal brain tissue. Furthemore the relationship between COX expression levels and cancer pathological grades was analyzed. RESULTS: Using RTPCR, all 20 fresh specimens from human astrocytomas were shown to express COX2 mRNA, while no positive expression of COX2 mRNA was found in 8 specimens of normal brain tissues; there was a significant difference between the two(Plt;0.0001). But COX1 mRNA was expressed constitutionally in all 20 of the fresh cancer tissues and 8 samples of normal brain tissues. The immunohistochemical result showed that COX2 protein was mainly localized at cytoplasm of malignant cells(positive rate was 72%), but was not expressed in normal brain tissues. In contrast, COX1 protein was mainly localized at brain cells in normal brain tissue(positive rate 100%), but was not expressed in human astrocytomas. In 50 paraffin embedded specimens of human astrocytomas, the intensity of COX2 expression was associated with tumor grade and stage. The level of COX2 expression was higher in grade Ⅲ, Ⅳ tumors than in grade Ⅰ, Ⅱ tumors. CONCLUSION: The expression of COX2 mRNA and protein is enhanced in human astrocytomas and is associated with malignant degree of the tumor,indicating that COX2 may play an important role in the development and progression of human astrocytomas. 　　【Keywords】 astrocytoma; prostaglandinendoperoxide synthase; reverse transcriptasepolymerase chain reactio