急性脑缺血大鼠模型血瘀证舌象表现评价方法及其及血液流变学.docVIP

急性脑缺血大鼠模型血瘀证舌象表现评价方法及其及血液流变学 　 作者：魏海峰, 亚白柳, 赵玲, 叶翠飞, 张丽, 李林 【关键词】 血瘀; 脑缺血; 再灌注; 血液流变学; 大鼠 Objective: To study the tongue tissue blood oxygen saturation measurement for evaluating tongue manifestation of blood stasis syndrome, and to explore its correlation with blood rheological disorder in a rat model of acute transient brain ischemia. Methods: Twenty-eight SD rats were randomly divided into sham-operated group and ischemia group. Middle cerebral artery occlusion was induced by thread in rats of the ischemia group. Tongue tissue blood oxygen saturation, neurological severity score and the changes of blood viscosity, red blood cell deformity, thrombin time and fibrinogen in the rats were measured after 24-hour reperfusion. Results: Blood viscosity and the content of fibrinogen in the ischemia group were significantly higher than those in the sham-operated group. Red blood cell deformity, thrombin time and tongue tissue blood oxygen saturation in the ischemia group were decreased as compared with the sham-operated group. There was a positive correlation between red blood cell deformity and tongue tissue blood oxygen saturation. Conclusion: Tongue tissue blood oxygen saturation is a good measurement for evaluating blood stasis in a rat model of focal cerebral ischemia, and this model can be used as a rat model of stroke with blood stasis syndrome. Keywords: blood stasis; brain ischemia; reperfusion; hemorheology; rats 理想的血瘀证动物模型对深入研究血瘀证生物学基础以及活血化瘀中药有重要意义［1］。以往评价动物模型的血瘀证表现主要通过血液流变学检测来实现，缺乏从模拟临床的宏观症状和体征等角度进行评价的手段。脑缺血再灌注大鼠模型常用来评价脑卒中治疗药物的疗效，我们近期的研究发现该模型的全血黏度、红细胞聚集率和血浆纤维蛋白原含量明显增高。本研究拟借助一种可测量组织血氧饱和度的仪器测定该模型大鼠的舌血氧饱和度，从宏观角度评价血瘀证的舌象表现，并和血液流变学异常表现进行相关分析，评价该方法作为观察血瘀证动物舌象变化指标的合理性，并进一步验证急性脑缺血大鼠模型的血瘀证特征。 1 材料与方法 1.1 主要试剂与仪器 聚乙烯吡咯烷酮，配制红细胞变形液，Sigma公司产品；10%水合氯醛，由首都医科大学宣武医院提供；栓线由北京沙东生物技术公司提供，使用前经0.1%多聚赖氨酸涂抹并在60 ℃下烤1 h［2］。 LBY-N6B全自动清洗血流变仪和微量血浆测试装置及LBY-BX3红细胞变形仪，均由北京普利生仪器有限公司提供；DL-4000B冷冻离心机，由上海安亭科学仪器厂生产；ViOptix公司发明的ODISseyTM局部组织血氧监护仪，由北京中沃科技有限公司提供。 1.2 动物及分组 28只SD大鼠，雄性，体质量（25