核因子κB、整合素αⅤβ3及卵巢癌血管生成关系探究.docVIP

核因子κB、整合素αⅤβ3及卵巢癌血管生成关系探究 　【关键词】 血管生成 　 【摘要】 目的 研究核因子κB（nuclear factor κB，NFκB）、整合素αⅤβ3与人卵巢上皮性癌(human ovarian epithelial carcinoma,hOEC)血管生成的关系。方法 原发性人卵巢上皮癌（hOEC）36份、卵巢良性肿瘤及正常卵巢各15份，应用免疫组化方法（ABC法）检测NFκB、整合素αⅤβ3、微血管密度（micro vessel density,MVD）在hOEC、卵巢良性肿瘤及正常卵巢组织中的表达。结果 NFκB、αⅤβ3和MVD在 hOEC中的阳性表达率分别为7222%、6667%和（4398±3373）%，在良性肿瘤组织中的表达率分别2667%、667%和（1308±907）%；在正常卵巢组织中的表达率分别为1333%、0和（710±542）%，hOEC组各指标的阳性率均显著高于良性卵巢肿瘤组及正常对照组（Plt;005）。hOEC中NFκB、αⅤβ3与MVD的相关系数分别为06203、06661，各指标与MVD均显著相关（Plt;001）。NFκB阳性表达者αⅤβ3的阳性表达率为8571%，显著高于NFκB阴性表达者αⅤβ3的表达率（为25%）（Plt;005）。结论 NFκB、αⅤβ3在hOEC中高表达与其血管生成密切相关，NFκB可能通过上调αⅤβ3表达而起核心调节作用。 【关键词】 核因子κB；整合素αⅤβ3；人卵巢上皮癌组织；血管生成 　　The study of relationships between nuclear factor κB,integrin αⅤβ3 and the angiogenesis of ovarian cancer 　　【Abstract】 Objective To study the relationships between （nuclear factor κB，NF κB）,integrin αⅤβ3 and the angiogenesis of human ovarian epithelial carcinoma (hOEC). Methods 36 hOEC,15 ovarian benign tissues and normal ovary tissue were selected,and the expressions of NFκB,integrin αⅤβ3 and MVD in hOEC,benign and normal ovarian tissue groups were detected by immunohistochemical staining (ABC method).Results　The expression rates of NFκB, integrin αⅤβ3 and MVD in hOEC were 72.22%,6667% and (4398±3373)%,respectively,which were 2667%,667% and (1308±907)% in benign tumor and were 1333%,0 and (710±542)% in the normal ovarian tissue.The expression rates of NFκB,integrin αⅤβ3 and MVD in hOEC were higher significantly than those in ovarian benign tumor and normal ovarian tissue.The correlation coefficient between NFκB,integrin αⅤβ3 and MVD were 06203 and 06661 respectively. There were significant relationship between NFκB,bFGF and MVD(Plt;001).The expression of integrin αⅤβ3 in NFκB positive group were 8571%,higher significantly than that in NFκB-negative group.Conclusion There were significant correlation between the high expressions of NFκB,integrin αⅤβ3 and the angiogenesis in hOEC,NFκB may play the key role by up regulate the e