水飞蓟宾对人膀胱癌细胞株5637增殖抑制作用及其机制.docVIP

水飞蓟宾对人膀胱癌细胞株5637增殖抑制作用及其机制 　 作者：孙羿　曾津　张栋　王新阳　朱国栋　祝广峰　程鹤鹏　贺大林 【摘要】 　　目的 研究水飞蓟宾(Silibinin, SB)在体外对人膀胱癌细胞株5637增殖的抑制作用及其相关机制。方法 采用MTT法观察不同浓度SB对人膀胱癌细胞株5637增殖的抑制作用；倒置显微镜下观察细胞形态变化；RTPCR检测凋亡抑制因子Survivin的mRNA表达变化。结果 MTT检测发现：随着SB浓度增加和作用时间延长，对人膀胱癌细胞株5637增殖的抑制作用增强，且各组间比较均有显著性差异(Plt;0.01)，并呈时间剂量依赖关系；倒置显微镜发现应用SB干预后，5637细胞体积缩小，变形，胞浆浓缩，核固缩深染；人膀胱癌细胞株5637中Survivin的mRNA表达随着SB浓度增加和时间延长相应下降。结论 SB可抑制人膀胱癌细胞株5637的增殖；并下调凋亡抑制因子Survivin的mRNA表达。 【关键词】 水飞蓟宾；癌；膀胱癌；增殖；survivin；人膀胱癌细胞株5637 　　Effect of silibinin on proliferation of human bladder cancer cell　5637 in vitro and related mechanism 　　ABSTRACT: Objective To determine the effects of silibinin on inhibiting the proliferation of 5637 cell in vitro and to investigate the related mechanism. Methods The cell growth activity was evaluated by MTT assay. Morphological changes were studied through the inverted phase contrast microscope. The expression of survivin(inhibitor of apoptosis protein) mRNA was determined by reverse transcriptionpolymerase chain reaction (RTPCR). Results Silibinin treatment of 5637 cells resulted in a moderate to strong growth inhibition in both a dose and a timedependent manner. There were significant differences between these groups (Plt;0.01). These morphological changes of 5637 which were treated with silibinin: cells shrank and turned round, condensed cytoplasma and pycnosised nucleus were observed through the inverted phase contrast microscope. There was also significant difference in the positive expression of survivin mRNA between the 5637 cell treated by differentiated silibinin. Conclusion Silibinin could inhibit the growth of human bladder cancer 5637 cells and downregulate the expression of survivin. 　　KEY WORDS: silibinin; carcinoma; bladder cancer; proliferation; survivin; 5637 cell line 　　水飞蓟素(SM)是从菊科植物水飞蓟中提取的黄酮类化合物，其组份中水飞蓟宾(silibinin, SB)含量最多，生物活性也最强。已知SB具有抗自由基活性、抗脂质过氧化、抑制5脂氧合酶等多种作用。近几年来研究还发现SB对各种上皮源性的肿瘤如前列腺癌、结肠癌、膀胱癌、肺癌等均具有良好抑制作用，并且已进入治疗前列腺癌的I期临床试验，但关于SB对膀胱癌的作用，国内外研究甚少。本文将研究SB在体外对人膀胱癌细胞株5637的抗增殖作用