纳米碳富勒烯抗I型人类疱疹病毒药效实验探究.docVIP

纳米碳富勒烯抗I型人类疱疹病毒药效实验探究 　［摘要］ 目的 观察富勒烯抗HSV-1的药效。方法 以逆相蒸馏法合成了富勒烯脂质体，用药物对病毒所致细胞病变的抑制实验以及病毒的空斑减数实验，观察C60脂质体对HSV-1F株的直接杀灭、感染阻断及增殖抑制作用。结果 在Vero细胞系统中，C60在光照及避光条件下，对HSV-1F株均具有直接杀灭和感染阻断作用，不具有增殖抑制作用。在光照条件下，对HSV-1F株直接杀灭的ED50、ED90分别为0.05313 μg/ml、0.1696 μg/ml；感染阻断的ED50、ED90依次为0.9461 μg/ml、5.643 μg/ml。在避光条件下，对HSV-1F株直接杀灭的ED50及ED90分别为12.90 μg/ml和26.20 μg/ml；感染阻断的ED50和ED90依次为24.13 μg/ml以及32.06 μg/ml。光照1 h，C60脂质体对Vero细胞的细胞毒为154.9 μg/ml。C60脂质体浓度为16.86 μg/ml及33.72 μg/ml时，对HSV-1F株直接杀灭的空斑减数率与光照时间成正相关，相关系数r分别为0.8510（Plt;0.005）和0.9766（Plt;0.005）；当C60脂质体浓度为16.86 μg/ml时，对HSV-1F株感染阻断的空斑减数率与光照时间正相关，相关系数r依次为0.84（Plt;0.005）及0.9923（Plt;0.005），说明C60的抗病毒作用具有时间效应，主要是光敏动力学机制起作用。结论 在光照条件下，C60对HSV-1F株直接杀灭及感染阻断的治疗指数分别为2915和163.7，C60抗HSV的作用值得进一步研究。 ［关键词］ 富勒烯C60；I型人类单纯疱疹病毒；抗病毒 Effect research of antiherpes simplex virus type-1 of nanometer carbon fullerene C60 liposome ［Abstract］ Objective To observe the effects of antiherpes sumplex virus type-1 with fullerene C60.Methods We prepare the fullerene C60 liposomes using reversed phase distillation method，examine the direct virucidal activity，infection block and multiplication inhibition of C60 liposomes to HSV-1F strain，using inhibition experiment of cellular pathologic effect of virus and plaque reduction assay.Results In Vero cell system，under lucifugal and illumination conditions，fullerene C60 has direct virucidal activity and infection block effect，but it does not has any multiplication inhibition action to HSV-1F strain.Under illumination condition，the ED50 and ED90 of direct virucidal activity of C60 to HSV-1F strain are 0.05313 μg/ml and 0.1696 μg/ml respectively，and the ED50 and ED90 of infection block action of C60 are 0.9461 μg/ml and 5.643 μg/ml separately.Under lucifugal condition，the ED50 and ED90 of direct virucidal activity of it to HSV-1F strain are 12.90 μg/ml and 26.20 μg/ml separately，the ED50 and ED90 of infection block action of it to HSV-1F strain are 24.13 μg/ml and 32.06 μg/ml respectively.After light radiation time of one hour，th