采用组织芯片技术探究宫颈癌中HPV及细胞周期调控因子p27表达关.docVIP

采用组织芯片技术探究宫颈癌中HPV及细胞周期调控因子p27表达关 　 作者：陈丽霞 沈燕如 罗文群 赖云英 罗斌华 刘勇 【摘要】 目的 研究宫颈癌组织中HPV感染与细胞周期调控因子p27表达的关系及意义。方法 采用组织芯片技术制作60例宫颈癌组织芯片，同时用S-P免疫组织化学和原位分子杂交方法检测宫颈癌组织芯片中HPV、p27、p27 mRNA的表达。结果 60例宫颈癌组织中HPV、p27、p27 mRNA的阳性率分别为48.3%、38.3%、51.7%。淋巴结转移阳性宫颈癌中p27和p27 mRNA阳性率明显低于淋巴结转移阴性宫颈癌（P＜0.01）。淋巴结转移阳性宫颈癌中HPV阳性率明显高于淋巴结转移阴性宫颈癌（P＜0.05）。宫颈癌和CIN Ⅲ级宫颈上皮内瘤变组织中HPV阳性率明显高于CINⅠ级和CINⅡ级（P＜0.05）。而p27和p27 mRNA阳性率明显低于CINⅠ级和CIN Ⅱ级宫颈上皮内瘤变组织（P＜0.05）。宫颈癌中HPV与p27及p27 mRNA表达呈明显负相关（r=-0.574，P＜0.05；r=-0.612，P＜0.01）。结论 HPV感染和细胞周期调控因子p27低表达与宫颈癌密切相关。HPV感染与p27低表达有关。HPV和p27可作为评估宫颈癌预后的参考指标。应用组织芯片大规模高效检测临床组织样本是可行的，具有快速、方便、经济、准确的特点。 【关键词】 组织芯片 宫颈肿瘤 乳头状瘤病毒 细胞周期 p27 ［Abstract］ Objective To investigate the relationship between cell cycle regulation factor p27 and HPV in cervical cancer tissue.Methods Sixty tissue chips of cervical cancer were made by tissue chip technique,S-P immunohistochemistry and in situ hybridization were simultaneously used to determine the expression of HPV,p27 mRNA in every tissue chip.Results Positive rates of HPV,p27,p27 mRNA in 60 cervical carcinoma tissues were 48.3%，38.3% and 51.7% respectively .Cervical cancers that had no lymphatic metastasis were found to be significantly more likely to express p27 and p27 mRNA and showed lower positive rates of HPV than that had lymphatic metastasis （P＜0.01＆P＜0.05,respectively）.The tissue of cervical cancer and cervical intraepithelial neoplasia Ⅲ （CIN Ⅲ） showed obvious higher positive rate of HPV and lower positive rates of p27 and p27 mRNA than the tissue of CIN Ⅰ and CIN Ⅱ （P＜0.05）.The expression of HPV showed obvious reverse correlation with that of p27 and p27 mRNA in the tissue of cervical cancer（r=-0.574, P＜0.05;r=-0.612，P＜0.01）.Conclusion Low expression of p27 and HPV infection are affinitive with cervical cancer and the HPV infection is due to the low expression of p27.HPV and p27 can be regarded as reference index used to estimate the prognosis of cervical cancer. ［Key words］ tissue chip;cervical cancer;papillomavirus,human;cell cycle;