姜黄素对脂肪变性人肝HL-7702细胞乙酰CoA羧化酶mRNA表达影响.pdfVIP

Effects of Curcumin on mRNA expression of acetyl-CoA carboxylase in human hepatocyte Abstract Objective: To observe the effect of curcumin to triglyceride’s disposition and expression of acetyl-CoA carboxylase mRNA in normal and nonalcoholic induced fatty liver cell. Methods: The investigation was conducted by means of randomized controlled trial. To establish a model of hepatocyte steatosis, we incubate the normal human HL-7702 hepatocyte in RPMI-1640 complete medium with 10% (V/V) fetal bovine serum plus 10% (V/V) medical fat emulsion injection for 24 hours. The experiment is divided into 6 groups: The first group is normal hepatocytes; the second group is fatty change hepatocytes;from group 3 to group 6, different amounts of curcumin is added to the fatty changed hepatocytes, to make the concentration of curcumin to be12.5 ，25, 50 ,100µmol/l respectively. After each group is incubated for 24 hours, And observe the formation of cellular morphology and lipid droplets in the cells under oil red O stain and determin the intracellular triglyceride levels through high performance liquid chromatography (HPLC). The semiquantitative RT-PCR was used to detect the mRNA levels of acetyl-CoA carboxylase of the hepatocytes of each group. According to the descendent level of intracellular triglyceride, the dose-effect relationship curve was made out. Then the cells were exposed to curcumin at the 100µmol/L concentration for 6hr, 12hr, 24hr ，48hr respectively, observe the above-mentioned parameters and determine the best duration for curcumin to decrease int