昆明城郊中国树鼩群体线粒体DNA遗传多样性.PDFVIP

动 物 学 研 究 2011，Feb. 32(1): 17−23 CN 53-1040/Q ISSN 0254-5853 Zoological Research DOI ：10.3724/SP.J.1141.2011.01017 Genetic diversity and matrilineal structure in Chinese tree shrews inhabiting Kunming, China CHEN Shi-Yi 1, XU Ling 1,3, LÜ Long-Bao 2, YAO Yong-Gang 1,* (1. Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences Yunnan Province, Kunming Institute of Zoology, Kunming Yunnan 650223, China; 2. Experimental Animal Core Facility Kunming Primate Research Center, Kunming Institute of Zoology , the Chinese Academy of Sciences, Kunming Yunnan 650223, China; 3. Graduate School of the Chinese Academy of Sciences, Beijing 100049, China) Abstract: Due to their special phylogenetic position in the Euarchontoglires and close affinity to primates, tree shrews have been proposed as an alternative experimental animal to primates in biomedical research. However, the population genetic structure of tree shrews has largely remained unknown and this has hindered the development of tree shrew breeding and selection. Here we sampled 80 Chinese tree shrews (Tupaia belangeri chinensis) in Kunming, China, and analyzed partial mtDNA control region sequence variation. Based on our samples and two published sequences from northern tree shrews (T. belangeri), we identified 29 substitutions in the mtDNA control region fragment (~ 604 bp) across 82 individuals and defined 13 haplotypes. Seventeen samples were selected for sequencing of the cytochrome b (Cyt b; 1134 bp) gene based on control region sequence variation and were analyzed in combination with 34 published sequ