番茄抗感青枯病品种的生理生化差异-浙江大学学报.pdfVIP

浙江大李李报(农业与生命科学版)31(5):550^554,2005 JournalofZhejiangUniversity(Agric.LifeSci.) 文章编号:1008-9209(2005)05-0550-05 番茄抗感青枯病品种的生理生化差异 寿森炎，冯壮志，殷一平，谭芸，苗立杯 (浙江大学 园艺系蔬菜研究所 生长发育与生物技术农业部重点实验室，浙江杭州310029) 摘 要:为了研究番茄杭青枯病育种的生理基拙，对差异很大的两个品种接种青枯病菌(Ralstonia solanacearum)，分析其品种间与防卫系统相关的动态变化.结果表明，受青枯菌侵染后，抗病品种 T51A的苯丙氛酸解氨酶(PAL)活性高于对照，而感病品种T9230的却与对照一致;抗病品种T51A的 超氧化物歧化酶(SOD)活性高于对照，其峰值比对照高16.12%，而感病品种T9230的却低于对照，其 峰值低于对照25.140o;杭病品种T51A与感病品种T9230的多酚氧化酶(PPO)、过氧化物酶(POD)活 性变化无明显差异;杭病品种T51A抑制叶绿素含量下降的能力及保持较高胡萝 卜素含量的能力明显 强于感病品种T9230. 关 键 词:番茄;品种;青枯菌;酶 中图分类号 :S641.2 文献标识码 :A SHOU Sen-yan,FENG Zhuang-zhi,YIN Yi-ping,TAN Yun,MIAO Li-xlang(Growthand biotechnologyofAgriculturalMinistryKeyLaboratory,DeptofHorticulture,ZhejiangUniversity, Hangzhou310029,China) Biochemicalandphysiologicaldifferencesbetweenresistantandsusceptibletomatocultivarsinfectedby RalstoniasolanacearumSmith.JournalofZhejiangUniversity(Agric.LifeSci.)，2005，31(5): 550-554 Abstract:Tostudythephysiologicalbasisoftomatobreedingforresistanttobacterialwilt,we inoculatedtheresistantandsusceptibletomatocultivarswiththepathogenbacterium torevealthe dynamicchangesintheactivitiesoffourenzymesinplantsofdifferentcultivarsinrelationtotheir resistance.Theresultsshowedthatthephenylanineammonialyase(PAL)activityofT51A，aresistant cultivar,washigherthanthatofthecontrol(CK)，whileforT9230,asusceptibleone,theactivitywas ataboutthesimilarlevelasthecontrol(CK)followingtheirinfectionbyRalstoniasolanacearum.The resistantcultivarexhibitedhigheractivityofsuperoxidedismutase(SOD)thanthecontrol(CK)，with thepeakvalueincreasedby16.12%.ButthesusceptiblecultivarwasinferiortotheCK,withthepeak valuedecreasedby25.14%o.Theactivityofperoxidase(POD)andpolyphenoloxidase(PPO)didnot differsignificantlybetweenresistantandsusceptiblecultivar.Thecapaci