国内外标记免疫分析技术研究现状（Current status of marker immunoassay in home and abroad）.docVIP

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国内外标记免疫分析技术研究现状（Current status of marker immunoassay in home and abroad）.doc

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国内外标记免疫分析技术研究现状（Current status of marker immunoassay in home and abroad）

国内外标记免疫分析技术研究现状（Current status of marker immunoassay in home and abroad） Current status of marker immunoassay in home and abroad 2010-03-07 10:06:55 source: Author: [small] read: 10 comments: 0 Abstract: gin=0 topMargin=0 labeled immunoassay is one kind of high sensitivity, high specificity of general detection technology, because of its unique advantages, has been widely used in various fields of basic and clinical medicine. Their basic principles are the same, and the signals are measured differently depending on the markings. Although the existing methods reported more than 10 species, but has a wide application value and has been widely used are: radioimmunoassay (RIA), enzyme immunoassay (EIA), time-resolved fluorescence immunoassay (TRFIA) and chemiluminescence immunoassay (CLIA). The following is a brief introduction to the present research status and future prospects of these four analytical techniques. Radioimmunoassay I. test tube solid phase method [1] At present, the direction of RIA development is to replace the conventional liquid phase with the test tube solid phase. In the solid phase method in antigen and antibody immune response after without adding separating agent and centrifugation procedures, only the measuring tube radiation can come to analyte concentration, the operation is convenient and fast, suitable for the detection of a large number of clinical samples. In particular, the use of washing instead of separation and centrifugation reduces non-specific binding and improves the accuracy and accuracy of the method. The solid phase antigen or antibody is coated with the conventional physical adsorption method, and the amount of the protein is small and the uniformity is poor, which is only suitable for double site sandwich method. In 1990, Causse et al. (2) proposed that polystyrene tubes could be activated by maleic anhydride two - styrene copolymer, and the binding stability, uniformity and protein content were improved by covalent binding.