如何制备透射、扫描电子显微镜样品（How to prepare samples for transmission and scanning electron microscopy）.docVIP

如何制备透射、扫描电子显微镜样品（How to prepare samples for transmission and scanning electron microscopy） How to prepare samples of transmission and scanning electron microscopy.Txt, too long to wait for most of the things that are already not what they wanted. A layer of a cool autumn rain, a valve and a fragrance, as time since unforgettable, a busy but nowhere to hide. Happy life nine words: there is hope, something to do, someone to love. Women and women as friends, to the attitude of the green leaves, but also hidden red flowers scheming. [how to prepare the sample of transmission and scanning electron microscope? Transmission electron microscopy (transmission electron microscope) and scanning electron microscopy (scanning electron microscope) the difference is: the former total magnification can vary in the range of 1000-1000000 times, the total magnification can change between 20-300000 times. Their preparation is as follows: equipment 1. strains Escherichia coli (Escherichia coli, Escherichia, coli) slope. 2. solution or reagent amyl acetate, concentrated sulfuric acid, anhydrous ethanol, sterile water, 2% phosphotungstic acid sodium (pH6.5-8.0) aqueous solution, 0.3% formaldehyde polyethylene (soluble in chloroform solution), cytochrome c, ammonium acetate, plasmid pBR322. 3. instruments or other appliances ordinary optical microscope, copper mesh, porcelain funnel beaker, dish, sterile dropper, sterile forceps, pins, slides, bacteria counting plate, vacuum coating machine, critical point dryer etc.. Three, operation steps (1) preparation and observation of samples by transmission electron microscopy 1. treatment of metal mesh Samples of the optical microscope were placed on the slides to be observed. But in transmission electron mirror, because the electron can not pierce through the glass, can only use the netted material as the carrier, usually called the carrier net. Because of the difference of materials and shapes, carrier network can be divided into many different