多通道在体记录技术——小鼠可推进式微电极阵列帽制作与植入手术.PDF

See discussions, stats, and author profiles for this publication at: /publication/259350307 [Multi-channel in vivo recording technique: Microdrive array fabrication and electrode implantation in mice]. Article in Sheng li xue bao: Acta physiologica Sinica] · December 2013 Source: PubMed CITATIONS READS 0 141 4 authors, including: Xiaoyu Ma East China Normal University 3 PUBLICATIONS 17 CITATIONS SEE PROFILE Some of the authors of this publication are also working on these related projects: hippocampal oscillations and corresponding interneurons View project All content following this page was uploaded by Xiaoyu Ma on 04 February 2016. The user has requested enhancement of the downloaded file. 生理学报Acta Physiologica Sinica , December 25, 2013, 65(6): 637–646 637 实验技术 多通道在体记录技术—— 小鼠可推进式微电极阵列帽制作与 植入手术 马晓宇，张艺瑶，王丽娜，林龙年* 华东师范大学脑功能基因组学教育部、上海市重点实验室，上海 200062 摘 要：本文旨在探讨适合小鼠在体记录的多通道可推进式微电极阵列帽的制作和电极植入手术。利用转动螺杆驱动螺母移 动的机械推进原理，用纤维板、螺杆、螺母和固定螺丝等材料，组装成可推进式多通道电极驱动装置。在该装置上，螺杆 转动一圈可使其上的螺母移动280 µm ，从而使固定于其上的电极也同步推进。记录电极为自制的四电极，由四股直径为13 µm的绝缘电极丝绞合而成。电极帽制作的主要步骤包括：四电极制作、电极驱动器制作、电极帽装配和电极镀金等步骤。 制作好的多通道电极帽重量轻，电极数量可根据实验需要设置，且可随时调整电极在脑内的深浅位置，适合在小鼠等动物 上开展多通道在体记录。此外，本文还详细介绍了在小鼠双侧海马进行多通道在体记录的电极植入手术过程。使用这种多 CA1 通道电极帽，可在自由活动的小鼠双侧海马 脑区，同时记录到群体神经元的放电活动。 关键词：多通道在体记录；四电极；小鼠；海马 中图分类号：Q424 Multi-channel in vivo recording technique: Microdrive array fabrication and electrode implantation in mice MA Xiao-Yu, ZHANG Yi-Yao, WANG Li-Na, LIN Long-Nian* Key Laboratory of Brain Functional Genomics (Ministry of Education and Shanghai Municipality), East China Normal University, Shanghai 200062, China Abstract: Here we describe and illustrate our methods for multi-channel in vivo recording in mice, including the