提取根系分泌物的方法,已引证.pdfVIP

J. Plant Nutr. Soil Sci. 2013, 176, 175–199 DOI: 10.1002/jpln.201000360 175 Review Article Methods of collection of plant root exudates in relation to plant metabolism and purpose: A review 1 1 3 2 1 Valerie Vranova , Klement Rejsek , Keith R. Skene , Dalibor Janous , and Pavel Formanek * 1 Department of Geology and Soil Science, Faculty of Forestry and Wood Technology, Mendel University in Brno, Zemedelska 3, 613 00, Czech Republic 2 ˇ CzechGlobe – Global Change Research Centre AS CR, v.v.i., Belidla 686/4a, 603 00 Brno, Czech Republic 3 Biosphere Research Institute, Letham, Angus DD8 2PY, Scotland Abstract The aim of this work is to review the current knowledge on the effects of plant metabolism (C , 3 C4, and CAM) on root exudation and on the methods of exudate collection as well as the use of such exudates for analyses, testing of microbial response, degradation of pollutants, enzymatic activities, and occurrence of allelochemicals. We examine the advantages and disadvantages of each method as related to the downstream use of the exudates. The use of continuous percola- tion of solid cultivation medium with adjustment of nutrient-solution strength appears to be a pro- mising methodology for the determination of root exudation rates and qualitative composition of exuded compounds. The method mimics rhizosphere conditions, minimizing the artificial accu- mulation of compounds, alteration of plasma-membrane permeability, ATPase activity, and the impacts of inhibitors or stimulators of root enzymes. Of particular significance is the fact that the adjustment of strength of nutrient solu