七味白术散对菌群失调腹泻小鼠乳酸杆菌多样性的影响.docVIP

七味白术散对菌群失调腹泻小鼠肠道乳酸杆菌多样性的影响* 郭抗萧1, 2 佘颜1 谢梦洲1 龙承星3 ** 谭周进1** 1湖南中医药大学 长沙 410208 2长沙卫生职业学院 长沙 410100 3 湖南人文科技学院数学与计量经济系 湖南 娄底 417000 摘 要：肠导微生物与人体多项生理功能相关，是中药药效发挥的重要作用者，研究七味白术散对菌群失调腹泻小鼠肠道乳酸杆菌的影响，可以为其疗效机理提供依据。通过灌胃硫酸庆大霉素和头孢拉定对小鼠进行肠道菌群失调造模，然后灌胃给药七味白术散全量传统汤药和50%量超微汤药对造模小鼠进行治疗，提取肠道微生物宏基因组DNA，用乳酸杆菌特异引物对肠道微生物的16S rDNA进行PCR扩增，通过Hha I、Rsa I、Sam I和Hind Ⅲ等酶切后进行ARDRA分析。结果发现正常组和七味白术散50%剂量超微汤药治疗组OTUs数大于七味白术散传统汤药治疗组，七味白术散对乳酸杆菌的多样性，均匀度及优势度均有所恢复，通过聚类与主成分分析表明，七味白术散对肠道乳酸杆菌具有一定的调整作用，且超微七味白术散50%量汤药的治疗效果优于七味白术散传统汤药。说明七味白术散对菌群失调腹泻的疗效与调整肠道乳酸杆菌有关，七味白术散50%超微汤药的效果比全量传统汤药的效果较好。 关键词：七味白术散；肠道微生物；ARDRA；菌群失调腹泻；乳酸杆菌 Lactobacilli Diversity of Intestinal Microbiota in Dysbacteria Diarrheal Mice Associated with Qiweibaizhusan* GUO Kangxiao1, 2,SHE Yan1, XIE Mengzhou1, LONG Cheng-xing3**, TAN Zhoujin1** 1 Hunan University of TCM, Changsha 410208, China 2 ChangSha Health Vocational College, Changsha 410100, China 3 Department of Mathematics and Econometrics, Hunan Institute of Humanities Science and Technology, LouDi 417000, Hunan Abstract：Gut microbes are closely associated with multiple human physiological functions, and play a significant role in the pharmacological effects of traditional Chinese medicine. This study is aim to investigate the microecological functions and their mechanism of ultra-micro powders of Qiweibaizhusan on the mice diarrhea with dysbacteriosis. The intestinal dysbacteriosis in mice were induced by orally administrating with gentamycin sulfate injection and cefradine capsules, and the effects of traditional decoction of Qiweibaizhusan and ultra-micro powders of Qiweibaizhusan (which dose is approximately equal to 50% dose of traditional decoction of Qiweibaizhusan) on the mice with diarrhea were respectively observed. The intestinal microbial metagenomics were extracted and then were PCR amplificated using lactobacilli specific primers. The diversity of PCR products were analyzed by using amplified rDNA restriction analysis (ARDRA). The results showed that the OTUs of l