甘草总黄酮对慢性浅表性胃炎大鼠胃粘膜损伤的保护作用.PDFVIP

J South Med Univ, 2013, 33(2): 299-304 doi 10.3969/j.issn. 1673-4254.2013.02.31 ·299 · 基础研究 甘草总黄酮对慢性甘草总黄酮对慢性浅表性胃炎大鼠胃粘膜损伤的保护作用浅表性胃炎大鼠胃粘膜损伤的保护作用 林晓春，陈育尧，白殊同，郑 君，佟 丽 南方医科大学中医药学院，广东 广州 510515 摘要：目的研究甘草总黄酮对慢性浅表性胃炎大鼠胃粘膜损伤的保护作用，探讨其相关药理机制。方法 采用0.02%氨水合并 饥饱失常刺激制备大鼠慢性浅表性胃炎模型，模型复制成功后动物随机分为模型对照组、维酶素对照组及甘草总黄酮高、中、低 剂量组，连续灌胃给药30 d。采用HE 染色观察胃组织病理学变化；阿利新蓝-糖蛋白结合法（AB-PAS）染色，检测胃粘膜上皮细 胞粘蛋白合成、分泌功能；ELISA 法测定血清中前列腺素E （PGE ）含量；胃体部、幽门部微循环血流量评价胃微循环状况。结 2 2 果 与正常对照组比较，浅表性胃炎模型组大鼠胃黏膜损伤率、胃组织病理学评分、粘蛋白含量显著改变；与模型对照组比较，试 药组血清PGE2 水平显著升高，上皮细胞粘蛋白指数及酸性粘蛋白指数提高，上皮细胞粘蛋白分泌功能增强和黏膜屏障完整性 得到恢复，大鼠胃黏膜损伤率显著下降。同时，胃微循环血流量改善。结论 甘草总黄酮抑制慢性浅表性胃炎大鼠胃粘膜损伤， 保护和修复损伤的粘膜组织，这种作用与提高PGE2水平相关。 关键词：甘草总黄酮；慢性浅表性胃炎；胃粘膜 Protective effect of licoflavone on gastric mucosa in rats with chronic superficial gastritis LIN Xiaochun, CHEN Yuyao, BAI Shutong, ZHENG Jun, TONG Li School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China Abstract: Objective To evaluate the protective effect of licoflavone on gastric mucosa in rats with chronic superficial gastritis and explore the possible mechanism. Methods SD rat models of chronic superficial gastritis was established by intragastric administration of 0.02% ammonia and long-term irregular diet. The rat models were then randomized into model group, vitacoenzyme group and 3 licoflavone groups of high, medium, and low doses. After 30 days of treatment, the gastric histopathology, mucosal lesions, scanning electron microscopy, mucin function production by the gastric mucosa epithelial cells, serum PGE2 level and gastric microcirculation were assessed to evaluate the protective effect of licoflavone on gastric mucosa. Results Compared with normal control rats, the rat models of chronic superficial gastritis showed significa