大孔树脂对刺葡萄籽中原花青素的纯化-湖南农业大学学报欢迎您！.pdfVIP

第 36 卷第 1 期 湖南农业大学学报( 自然科学版) Vol.36 No.1 2010 年 2 月 Journal of Hunan Agricultural University (Natural Sciences) Feb ．2010 大孔树脂对刺葡萄籽中原花青素的纯化 * a a a a b b 王辉宪 ，马玉美 ，罗启枚 ，谢聃 ，刘东波 ， 熊兴耀 (湖南农业大学 a.理学院；b. 园艺园林学院，湖南 长沙 410128) 摘 要：运用正交试验筛选树脂，探索原花青素在所选树脂上的吸附和解吸工艺条件．结果表明，所考察的 YWD-06C 、YWD-09F 、YWD-03K7 、YWD-04B1、HPD-300、HPD-700、D-101 和 DS-401 树脂中，YWD-06C 树 脂为葡萄籽原花青素纯化的最佳树脂；最佳吸附条件：吸附温度 20 ℃，吸附时间6 h ，溶液pH 值为 4 ；最佳解 吸条件：解吸液为 60%的乙醇水溶液，pH 值为 4 ，解吸时间6 h ，解吸温度25 ℃ ．通过动态试验，确定吸附速 率为 4 B/h ，洗脱速率为2 BV/h ，经该工艺所得纯化物中原花青素的含量达92.57% ． 关 键 词：刺葡萄籽；原花青素；大孔树脂；纯化 中图分类号：R284.2 ；S663.1 文献标志码：A 文章编号：1007-1032(2010)01-0039-06 Purification of proanthocyanidins from grape seeds by macroiporous adsorption resins a a a a b b* WANG Hui-xian , MA Yu-mei , LUO Qi-mei , XIE Dan , LIU Dong-bo , XIONG Xing-yao (a.College of Sciences; b. College of Horticulture and Landscape, HNAU, Changsha 410128, China) Abstract ：The technics of purification of proanthocyanidins from grape ( Vitis davidii Foëx) seeds were studied. Resin was chosen by orthogonal experiment, capability of adsorption and desorption and effect factors of proanthocyanidins on resins were reviewed, the best conditions for adsorption and desorption on the chosen resin of proanthocyanidins were researched. The result were as follows: the best chosen resin was YWD-06C, the optimal adsorption conditions were: temperature, 20 ℃, ads