大熊猫辅酶q-细胞色素c氧化还原酶的辅酶q连接蛋白基因cdna的.pdfVIP

兽类学报,2007,27 (2):190 - 194 Acta Theriologica Sinica 大熊猫辅酶 Q-细胞色素 C 氧化还原酶的辅酶 Q 连接蛋白基因 cDNA 的克隆及序列比较 1 1 1 1 2 侯万儒 陈瑜 彭正松 吴夏 唐宗祥 (1 西华师范大学生命科学学院, 南充 637002 ) (2 四川农业大学农学院,雅安 625014 ) 关键词:大熊猫;RT-PCR;辅酶 Q 连接蛋白基因;克隆 中图分类号:Q751 文献标识码:A 文章编号:1000 - 1050 (2007 )02 - 0190 - 05 cDNA cloning and sequences analysis of ubiquinol-cytochrome c reductase complex ubiquinone-binding protein (QP-C)from giant panda 1 1 1 1 2 HOU Wanru ,CHEN Yu ,PENG Zhengsong ,WU Xia ,TANG Zongxiang (1 College of Life Science,China West Normal University,Nanchong 637002 ,China) (2 College of Agriculture,Sichuan Agricultural University,Ya′an 625014 ,China) Abstract :In order to provide scientific material for promulgating the giant panda (Ailuropoda melanoleuca)nucleus gene inheritance characteristic,for the formulation gene protection countermeasure and to put resources to rational use,the ex- pression sequence of ubiquinol-cytochrome c reductase complex ubiquinone-binding protein (QP-C )gene from the giant panda was analyzed The result indicates that the cDNA sequence length is 335 bp and contains a 246-nucleotide open 3 reading frame encoding 81 amino acid residues The pI of the protein is 10 50 and its mole cular weight is 9 60 × 10 Da Topology prediction shows these QP-C proteins contain a N-glycosylation site,a potential protein kinase C phosphorylation site and a Casein kinase II phosphorylation site. Comparing the cDNA sequence with amino acid sequence of giant panda ’s QP-C gene and cDNA se