高效sirna设计的研究进展-遗传.pdfVIP

遗 传 HEREDITAS (Beijing) 28(11): 1457~1461, 2006 专论与综述 DOI: 10.1360/yc-006-1457 高效 siRNA 设计的研究进展 许德晖, 黄 辰, 刘利英, 宋土生 (西安交通大学医学院遗传学与分子生物学系/环境与疾病相关基因教育部重点实验室, 西安 710061) 摘 要: RNA 干扰(RNA interference, RNAi) 是生物界普遍存在的一种抵御外来基因和病毒感染的保守进化机制, 其本质是 siRNA 与靶向 mRNA 特异结合、并由 RISC 介导其降解, 从而阻止 mRNA 的翻译, 导致基因沉默。因此, RNAi 可以作为基因功能研究、基因治疗等的新工具。但是, 随机设计的 siRNA 之间沉默效应差别很大。如何针对 靶基因设计特异、高效的 siRNA 就成了一个关键的问题。文章对 siRNA 设计原则的研究进展进行了总结论述。 关键词: RNAi; siRNA; 序列设计 中图分类号: Q752 文献标识码: A 文章编号: 0253-9772(2006)11-1457-05 New Progress of the Highly Efficient siRNA Design XU De-Hui, HUANG Chen, LIU Li-Ying, SONG Tu-Sheng (Department of Genetics and Molecular Biology, College of Medicine of JiaoTong University/Key Laboratory of Environment and Dis- ease-Related Gene, Education Ministry, Xi’an 710061, China) Abstract: RNA interference (RNAi), a conserved evolutionary mechanism widely found in living nature, is considered for defending the alien gene and virus infection. RNAi can induce gene silencing by prohibiting the translation of the target mRNA, which is specially recognized by the corresponding siRNA, and finally degraded by the RISC. So RNAi can be applied as a new technology tool for the functional gene research and gene therapy. But the effects of random designed siRNAs on gene silencing differ greatly from each other. The design of the special, highly efficient siRNA comes to be a crucial problem. This issue gives an overview a