膀胱癌患者外周血源性树突状细胞表型及免疫功能-第三军医大学学报.docVIP

膀胱肿瘤患者外周血源性树突状细胞表型及免疫功能变化 刘 星，梁 平，周 波，张克勤，李彦锋 （400042 重庆，第三军医大学大坪医院野战外科研究所泌尿外科） [摘要] 目的 探讨膀胱肿瘤患者外周血源性树突状细胞（dendritic cells,DCs）的表型及免疫功能变化。方法 通过密度梯度离心法从膀胱肿瘤患者和正常人外周血分离单个核细胞，加rhGM-CSF和rhIL-4诱导培养树突状细胞，采用流式细胞仪检测2组DCs表达程序性死亡配体-1（PD-L1）、CD1a、HLA和CD83的变化，混合淋巴细胞反应检测其刺激T淋巴细胞增殖能力和ELISA法检测分泌IL-10和IL-12的变化。结果 膀胱肿瘤患者外周血DCs表达PD-L1[（95.06±4.06）% vs （76.63±6.90）%]和分泌IL-10[（214.00±13.75） pg/mL vs （83.78±7.95）pg/mL]的水平显著高于正常组（P0.05），DCs表达CD83[（16.20±1.91）% vs （35.53±1.58）%]及刺激淋巴细胞增殖的能力均低于正常组水平（P0.05）。结论 膀胱肿瘤患者外周血源性树突状细胞高表达PD-L1、低表达CD83及过多分泌IL-10可能是膀胱肿瘤发生免疫逃逸的原因之一。 [关键词] 膀胱肿瘤；树突状细胞；PD-L1 [中图法分类号]：R737.14 [文献标志码] A [基金项目] 国家自然基金 [通信作者] 靳风烁，E-mail:jinfs5505@163.com The changing in phenotype and metergasis of dendritic cells in peripheral blood of patients with bladder carcinoma Liu Xing , Liang Ping , Zhou Bo , Zhang Keqin , Li Yanfeng, Jin Fengshuo （Department Of Urology, Da-ping Hospital Research Institute of Field Surgery , Third Military Medical University , Chongqing, 400042, China） Abstract: Objective: To investigate the phenotype and metergasis of dendritic cells (DCs) in peripheral blood of patients with bladder carcinoma. Methods: DCs were isolated from the peripheral blood of patients with bladder carcinoma and healthy subjects by density gradient centrifugation, respectively, and then the two groups of DCs were cultured in vitrol with rhGM-CSF and rhIL-4 inducing. The expression levels of PD-L1,CD1a, HLA and CD83 expressed on DCs were detected by flow cytometer, the proliferation of lymphoid cells stimulated by the two groups of DCs were tested by mixed lymphocyte reaction, and the levels of IL-10 and IL-12 secreted by the two groups of DCs were measured by ELISA assay. Result: we found that the levels of PD-L1 expressed on DCs (95.06±4.06%vs76.63±6.90%) and IL-10 secreted by DCs (214.00±13.75 pg/mLvs83.78±7.95pg/mL) were significantly higher in the patients than in the controls, whereas CD83 expression levels (16.20±1.91%vs35.53±1.58%) was si