喹禾灵及喹禾灵酸在土壤中的分析方法的-中国生态农业学报.docVIP

喹禾灵对映体对斑马鱼毒性的手性选择性研究 许阳光1王鹏1张金玲1刘亮2李学锋1 中国农业大学北京100094; 2. 农业部农药检定所北京100024) 摘 要 为了研究喹禾灵对映体对水生生物的手性选择性毒性, 对喹禾灵对映体进行了斑马鱼急性毒性试验, 并研究了亚致死水平上对斑马鱼体内ATP酶活性的影响。研究发现, 精喹禾灵和喹禾灵对斑马鱼的急性毒性分别为高毒和低毒; 二者对斑马鱼体内Na+, K+-ATP酶和Ca2+-ATP酶活性无论在诱导时间和诱导强度上都表现出不同的作用方式。喹禾灵对斑马鱼Ca2+-ATP酶活性的影响在72 h时0.25 mg·L(1、1.00 mg·L(1两个暴露组表现出明显的诱导效应(P0.05), 在96 h各暴露组表现出了整体的诱导作用。精喹禾灵除24 h 5 μg·L(1、10 μg·L(1暴露组外, 其他各个暴露浓度组对斑马鱼体内Ca2+-ATP酶表现出较强的诱导作用。72 h之后, 25 μg·L(1、100 μg·L(1暴露组酶活性有下降的趋势, 其他2个浓度组酶活性则继续增加。喹禾灵对斑马鱼Na+, K+-ATP酶活性的影响在48 h时, 0.25 mg·L(1、1.00 mg·L(1两个暴露组对斑马鱼Na+, K+-ATP酶活性有明显的诱导效应(P0.05), 72 h时只有1.00 mg·L(1暴露组有明显诱导作用; 精喹禾灵25 μg·L(1、100 μg·L(1暴露组在24 hNa+对K+-ATP酶活性即表现出很强的诱导效果; 48 h后, 各个暴露组酶活性都开始明显的增加; 而在72 h, 各个染毒浓度组酶活力有下降的趋势, 96 h后基本与对照组无显著性差异。试验结果表明喹禾灵对映体对斑马鱼毒性有显著的手性选择性。 关键词quizalofop enantiomers toxic on Brachydanio rerio chiral selective XU Yang-guang1,WANG Peng1,ZHANG Jin-ling1, LIU-Liang2,LI Xue-feng1 (1.China Agricultural University, Beijing, 100094; 2. Institute for the Control of Agroche Micals of China, Beijing 100024) Abstract To study the chiral selective toxic of quizalofop enantiomers on the aquatic specials, acute toxicity test of quizalofop enantiomers to Brachydanio rerio has been conducted, and the variance of ATPase activity in Brachydanio rerio under sublethal concentrations is under investigation. It is found that quizalofop is highly toxic to Brachydanio rerio while quizalofop-p-ethy1 belongs to low-toxic pesticides. For Na+, K+-ATPase and Ca2+-ATP, modes of quizalofop enantiomers in both induction time and the induction intensity are different. As a result , At 24 h and 48 h the effect of quizalofop on the activity of Ca2+-ATP synthase for Brachydanio rerio was no significant difference between each exposure group and control group. At 72 h, the exposure groups 0.25 mg·L(1 and 1.00mg·L(1 showed significant induction (P0.05). At 96 h, each exposure group showed overall induction. Quizalofop-ethyl showed significant induction for Ca2+-ATP synthase for Brachydanio rerio in