壳聚糖没食子酸衍生物酶法催化条件优化及抗氧化活性和细胞毒性.pdfVIP

※工艺技术 食品科学 2017, Vol.38, No.02 227 壳聚糖没食子酸衍生物酶法催化条件优化及 抗氧化活性和细胞毒性 吴 昊，王艺颖，甄天元，罗 丹，张 潇，杨绍兰，王成荣* （青岛农业大学食品科学与工程学院，山东青岛 266109 ） 摘  要：利用漆酶、辣根过氧化物酶在均相和非均相反应体系中催化壳聚糖与没食子酸反应，以增强壳聚糖的抗氧 pH 化性。研究酶种类、反应体系 值、反应温度、反应时间、酶用量和底物的质量比等因素对产物接枝率的影响。 pH 4.5 25 5 h 利用单因素试验和正交试验确定的最适反应条件为反应 、反应温度 ℃、反应时间 、没食子酸与壳聚糖 3 1 4 U 65.2% 的质量比 ∶、漆酶用量 ，此条件下衍生物的接枝率为 。对衍生物分别进行体外抗氧化活性和细胞毒性检 测，结果表明，在相同添加量的情况下，衍生物的抗氧化性显著高于未改性的壳聚糖，而且产物无细胞毒性。 关键词：酶；壳聚糖没食子酸衍生物；抗氧化；细胞毒性 Optimization of Enzymatic Reaction between Chitosan and Gallic Acid for Enhanced Antioxidative Activity and Reduced Cytotoxicity * WU Hao, WANG Yiying, ZHEN Tianyuan, LUO Dan, ZHANG Xiao, YANG Shaolan, WANG Chengrong (College of Food Science and Engineering, Qingdao Agricultural University, Qingdao 266109, China) Abstract: In this paper, chitosan (CTS) grafted copolymer with gallic acid (GA) was synthesized by laccase and horse radish peroxidase to enhance the antioxidant activity of chitosan. The effects of enzyme type and dosage, initial reaction pH, temperature, reaction time, and enzyme-to-substrate ratio on the grafting ratio were studied. The maximum grafting ratio (65.2%) was obtained when the reaction took place at 25 ℃ for 5 h in 100 mL of acetate buffer solution (100 mmol/L) at pH 4.5 with a mass ratio of GA to CTS of 3 in the presence of 4 U of laccase under constant stirring. At the same dosage, the CTS- GA copo