中小负荷运动及慢性情绪应激对免疫机能影响.docVIP

中小负荷运动及慢性情绪应激对免疫机能影响摘要：为了寻找对抗情绪应激有效干预手段，维持机体免疫功能的稳定。发现：用54只SD雄性大鼠，随机分为七组进行实验研究。结果：与非应激组相比，应激组大鼠血清皮质酮含量显著升高，血清IL-2、CD3+CD4+CD8+水平显著下降；与非运动组相比，30min和60min运动组血清IL-2水平和血浆T细胞亚群数目都显著升高，而血清皮质酮含量显著降低。表明：运动训练可以通过改变HPA轴的应激激素的变化对抗心理应激造成的免疫功能的低下。 关键词：心理应激；运动；T淋巴细胞亚群；皮质酮白细胞介素2 中图分类号：G804.7 文献标识码：A 文章编号：1007-3612(2007)07-0919-03 The Effects of Low and Moderate Intensity Exercises and Psychological Stress on Immune System XIAO Chun-mei1，YIN Lin2 (1.Beijing Institute of Graphic Communication, Beijing 102600, China；2.Zhoukou Vocational Technical College,zhoukou 466001,Henan China) Abstract:To find the kind of intervene measures which could be used to antagonize the stress and keep the stability of one’s immune system, 54 SD rats were divided into 7 groups in the experiment. Compared with the control group, the IL-2 level and the number of CD3+ and CD4+/CD8+ of psychological stress group was significantly lower than that of control group, while their corticosterone level was significantly higher than that of control group. Compared with the no exercise group, the IL-2 and CD3?+,CD4?+/CD8?+ levels of both 30min group and 60min group were significantly higher than that of control group, while their corticosterone level was significantly lower than that of control group. The effects of exercises are likely related to the adaptation of HPA following chronic. Key words: psychological stress; exercise; T lymphocyte subsets; corticosteroneIL-2 长期情绪应激状态下可以通过神经内分泌系统，中枢神经系统影响机体免疫功能，危害人体的健康。采用何种干预手段对抗情绪应激从而维持机体免疫功能的稳定成为现代心理神经免疫学研究的热点。身体锻炼作为一种适合现代人的健身、健心的方法，关于身体锻炼对纯心理情绪应激下的免疫功能的变化及其机制的研究甚少。本研究旨在观察中小负荷运动与慢性情绪应激对免疫的影响，通过对运动大鼠血清皮质酮、IL-2和血浆T淋巴细胞亚群的检测，从免疫学和神经内分泌学角度探讨这种有益身心作用的可能机制。 1材料与方法 1．1实验动物 SD雄性大鼠54只，鼠龄7周，体质量(201.29±10.75)g，健康普通级，由北京医科大学实验动物中心提供，大鼠购回后，适应性饲养一周，剔除不合格动物后将大鼠随机分为为7组，即:对照组、电击组、心理应激组、60min训练组、30min训练组、应激+60min训练组、应激+30min训练组，每组7～8只。分笼饲养，各组大鼠实验前标记，每天称重量。整个实验过程自由进食饮水，采用国家标准固体混合饲料喂养大鼠每天7:00和18:00喂食两次，环境温度控制在20±2℃,湿度45%，光照时间12h(7:00-19:00)。 1．2实验方法