产褐藻胶裂解酶菌株cobetia sp.wg-007的筛选及发酵优化 screening of alginate lyase producing strain cobetia sp.wg-007 and optimization of fermentation.pdfVIP
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产褐藻胶裂解酶菌株cobetia sp.wg-007的筛选及发酵优化 screening of alginate lyase producing strain cobetia sp.wg-007 and optimization of fermentation
产褐藻胶裂解酶菌株Cobetia
sp.
WG一007的筛选及发酵优化
刘旭梅, 李恒, 蒋敏, 龚劲松, 许正宏, 史劲松4
(江南大学药学院,江苏无锡214122)
摘要:以褐藻酸钠为唯一碳源,从腐烂的海带中筛选出一株高效降解褐藻胶的菌株,经形态、生
理生化和16SrDNA测序鉴定,将其命名为Cobetia
sp.WG一007。采用单因素和JY_交试验优化,
20
确定该菌株摇瓶发酵最适产酶条件为褐藻酸钠6.0g/L,蛋白胨5.0g/L,NaClg/L、KzHPO。
0.25 h达到最高酶活,为160.7U/mL,相比优化前酶活力提
g/L,菌株在25℃条件下发酵培养30
高了3.52倍。
关键词:褐藻胶裂解酶;Cobetia
sp.WG一007菌株;筛选;发酵
920.1
中图分类号:TO 文献标志码:A 文章编号:1673--1689(2014)09—0903—07
of StrainCobetia and
ScreeningAlginateLyaseProducing sp.WG一007
ofFermentation
Optimization
LIU Min,GONG
Xurrtei,LIHeng,JIANG Jinsong,XUZhenghong,SHI)insong
ofPhamlaceutical
(School University,Wuxi214122,China)
Science,Jiangnan
Abstract:Aefficient strainwasisolatedfromrottenseaweedsodium
high alginate-degrading using
asthesolecarbonsource.Basedonthe characteristicsaswellas16SrDNA
alginate physiological
strainwasidentifiedasCobetia fermentationmedium
optimized
sequencing.the sp.WG一007.The
was ofsodium 6.0 5.0 20 0.25
composed alginateg/L,peptoneg/L,NaClg/L,K2HP04g/L.The
strainwascultivatedat25ocfor30hunderthe conditionsandthe
optimized alginatelyaseactivity
wasincreasedto160.7U/mL.whichwas3.52一fold withthecontr01.
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