万寿菊花中α-三连噻吩的提取及抑菌作用研究 study on extracting condition and anti-bacterial activity of α-terthienyl from marigold.pdfVIP

现代食品科技 Modern Food Science and Technology 2011, Vol.27, No.3 万寿菊花中 α-三连噻吩的提取及抑菌作用研究 王宪青，岳茹冰，王秋月 （黑龙江八一农垦大学食品学院，黑龙江大庆 163319） 摘要：以万寿菊花为原料，用氯仿提取其中光活化杀虫物质α-三连噻吩，并利用气相色谱测定所提取液中 α-三连噻吩的含量。 通过正交实验研究了料液比、时间、温度等因素对 α-三连噻吩得率的影响，实验表明，最佳提取工艺参数为料液比为 1:20、提取时 间为5 h、温度85 ℃，提取率达到 1.5042%。最佳条件下得到的万寿菊提取液对青霉、大肠杆菌和枯草芽孢杆菌具有抑菌作用，48 h 抑制率分别为 2.99%、2.43%和4.18% ，最小抑菌浓度为 15%，此时α-三连噻吩的浓度为 0.38%。 关键词：α-三连噻吩；气相色谱；抑菌 文章篇号：1673-9078(2011)3-299-302 Study on Extracting Condition and Anti-bacterial Activity of α-terthienyl from Marigold WANG Xian-qing, YUE Ru-bing, WANG Qiu-yue (Food College Heilongjiang August First Land Reclamation University, Daqing 163319) Abstract: The marigold flower was used to extract photoactivited pesticide α-terthienyl with chloroform, and the α-terthienyl extracted was measured with gas chromatography. Through orthogonal experiments the influence on the yield of α-terthienyl by factors such as the ratio of material to solvent, extracting time and temperature was studied. The experiment showed that the optimal condition is that the ratio of material to solvent is 1:20, the extracting time is 5 h and the extracting tempreture is 85℃, and the the yield of α-terthienyl reached 1.5042%.The extracts in optimal condition could inhibit the growth of penicillium, coliform and bacillus subtilis. and the inhibitory rate was 2.99%, 2.43% and 4.18% after 48h. The minimum inhibition concentration of extracts was 15%, and the concentration of α-terthienyl was 0.38%. Key words: α-terthienyl; gas chromatography; anti-bacterial 万寿菊，俗称臭芙蓉，金盏花，菊科万寿菊属， 目前对α-三连噻吩的研究多集中在对其光化学活 一年生草本植物。夏秋季开花，花呈黄色或橙色，易 性的研究，对其提取工艺没有一个确定的研究方案， 于栽培。原产墨西哥，由于其花具有花形较大、绚丽 并且将气相色谱法直接应用于针对万寿菊中α-三连噻