PKM2 siRNA对人甲状腺乳头状癌细胞生长和增殖的影响.pdfVIP

278 刘 睿 ，等 PKM2alRNA对人甲状嚏乳头状癌细胞生长和增殖的影响 10] CaoW WanY LiangZH etalHearedlipiodolasanembo]iza 0IVX2livercancer[J]EurJRadiol，2010，73(2)：412 419 tiona~ent r*lanshepaticarterialembolizadon lnalabbitmodel (编校 ：徐萌) PKM2siRNA对人甲状腺乳头状癌细胞生长和增殖的影响 刘 睿 ，王晓楠 ，张 薇。，关海霞 EffectofPKM2siRNAongrowthandproliferationofpapillarythyroidcancercel LiuRui，WangXiaona ，ZhangWei，GuanHaixia Department Endocritu)log?,andMetabolism，TheEndocrineInstituteand TheLiaoningProvincial1eyLaboratory。，EndocrineDisea ㈣ ，‰ FirstHospital ChinaMedicalUniversity，LiaoningShenyangl10001，China；Department Geriatrics，TheFirstHospital。， ChinaMedicalUniversity，LiaoningShenyang110001，China；DepartmentofEndocriru)log?,andMetabolism，ShengfingHospital。}China MedicalUniversity，Liaoning Shenyang 110011，China 【Abstract】 Objective：ToinvestigatetheexpressionpatternofPKM2inpapillat3~thyroidcancer(PTC)andthe possibleroleofPKM2inPIC M ethods：grCcallsweretransfeetedwithsmallinterferingRNA againstPKM2 PKM2 expressionincellsWSSanalyzedbyWesternblotandRT—PCR CellgrowthandproliferationWSSevaluatedby MTI assaysandcolonyformationassaysLactateandATPproductionandglucoseconsumptionbycellsweredeterminedu— singcommercialassay kits Results：PKM2wasaberrantly overexpressed in PTC Specific siRNA againstPKM2 in PIC celllinesretardedcallgrowthanddecreased theircolony—formingcapabliity PKM2 knockdown alsoreduced lactateandATPproduction，andglucoseconsumptionbygrCcellsConclusion：OverexpressionofPKM2providesa selectivegrowt hadvantageforPICcellsthroughactivationofglycolysisAberrantPKM2overxepressionmayserve88 anovelbiomarkerand potentialtreatmenttargetforPTC 【Keywords】papillarythyroidcancer；PKM2；siRNA；glycolysis ModernOneology2014，22(02)：0278—0282 【摘要】 目的：研究PKM2在 甲状腺乳头状癌(PTC)中的表达特性及其在