紫薯中绿原酸类化合物标准品的制备-成都曼思特生物科技有限公司.pdfVIP

※成分分析 食品科学 2015, Vol.36, No.12 133 紫薯中绿原酸类化合物标准品的制备 1 1 2 1 李 甫，陈 斌 ，李联洪，王明奎 （1.中国科学院成都生物研究所，四川 成都 610041 ；2.成都曼思特生物科技有限公司，四川 成都 610000 ） 摘  要：从紫薯中提取制备高纯度、市场价值较高的绿原酸类化合物标准品。紫薯切片，采用沸水提取、大孔树脂 吸附和凝胶柱色谱分段，然后以反相制备液相色谱获得高纯标准品，最后以核磁共振技术确定化学结构。从紫薯中 A B C 3,4,5-O- 制备得到异绿原酸 、异绿原酸 、异绿原酸 和 三咖啡酰基奎宁酸，高效液相色谱检测表明这些化合物的 98% 纯度均在 以上。本研究建立的制备工艺可高效地将紫薯中绿原酸类化合物富集，并快速获得高纯样品，有效地 避免了紫薯花色苷类化合物对产品色泽和纯度的影响。 关键词：紫薯；绿原酸类化合物；标准品；分离；制备 Preparation of High-Purity Chlorogenic Acid Derivatives from Purple Sweet Potato 1 1 2 1 LI Fu , CHEN Bin , LI Lianhong , WANG Mingkui (1. Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China; 2. Chengdu MUST Bio-technology Company Limited, Chengdu 610000, China) Abstract: The purpose of the present study was to prepare high-purity and high-value chlorogenic acid derivatives from purple sweet potato. Purple sweet potato slices were extracted with hot water. The extracted compounds were separated with D72 macroporous resin, sephadex LH-20 column chromatography and preparative high performance liquid chromatography (HPLC) to obtain high-purity chlorogenic acid analogues which were identified as isochlorogenic acid A, isochlorogenic acid B, isochlorogenic acid C and 3,4,5-O-tricaffeoyl quinic acid, respectively, by nuclear magnetic resonance technique. HPLC analysis suggested that the purities of these analogues were all over 98%. The developed method can be used to rapidly obtain standard samples of chlorogenic acid derivatives from purple sweet potato, while