混合菌同步糖化共发酵造纸污泥产乙醇 the production of ethanol from paper mill sludge by simultaneous saccharification and fermentation of mixed bacteria.pdfVIP

Technology ·技术进步 The production of ethanol from paper mill sludge by simultaneous saccharification and fermentation of mixed bacteria a,b a,b a a a ⊙ WANG Ming-sheng , HE Gang , CHEN Jie-nan , ZHAN Peng , ZHANG Wei-tao (a.Institute of Biologi - cal Environmental Science Technology; b.College of Life Science and Technology, Central South University of Forestry and Science, Changsha 410004, China ) 混合菌同步糖化共发酵造纸污泥产乙醇 a,b a,b a a a ⊙ 王明生 何钢 陈介南 詹鹏 张伟涛 （中南林业科技大学 a.生物环境科学与技术研究所； b.生命科学与技术学院，长沙 410004） 摘 要： 对酿酒酵母（Saccharomyces cerevisiae）与重组大肠杆菌KO11(Escherichia coli)混合 菌同步糖化共发酵造纸污泥产乙醇进行了初步研究。在底物浓度为50g/L时，通过单因素 实验和正交实验获得乙醇发酵的最佳条件：纤维素酶添加量25FPU/g底物，接种量为6%， 酿酒酵母与重组大肠杆菌KO11接种比例为1∶1（细胞干重初始浓度分别为1.0g/L和0.3g/L 左右）。发酵72h后，乙醇浓度为5.71g/L，产率达到0.114g乙醇/g污泥，达到理论值的 42.3%。分别用酿酒酵母、KO11单菌种发酵与双菌株组合发酵对比结果表明，混合菌发酵效 果明显优于单菌种发酵。 关键词： 造纸污泥；混合菌；同步糖化共发酵；乙醇 Abstract: A primary study on mixed bacteria simultaneous saccharification and ethanol fermentation of paper mill sludge of saccharomyces cerevisiae and recombinant Escherichia coli KO11 was carried out. The results show that when the substrate concentration is 50g/l, through the single factor and orthogonal experiments the best conditions of ethanol fermentation are got as follows: the amount of 王明生,硕士研究生；研究 cellulase 25 FPU/g substrate, inoculation 6%, and the reorganization of the large intestine of saccharo- 方向：生物化学与分子生