高效液相色谱法测定食品接触材料中苯甲醛的迁移量.PDFVIP

PT CA( PART B: CH EM . ANA L. ) 20 11 47 5 , , , , , , , ( , 5 18045) : 选择4 种模拟食品的介质, 对其从食品接触材料( 例如聚苯乙烯) 中迁移的苯甲醛量用 高 液相色谱法( HPLC) 作了测定将聚苯乙烯样品分别与3 种模拟水基食品介质 一起在 95 回流2 h, 将所得2 份提取液分别用相同介质定容为100 mL 后供HPLC 分析另将聚苯乙烯样品 置于密闭的具塞锥形瓶中, 用葵花籽油作为模拟脂基食品介质, 在95 浸泡2 h, 冷却后称取此油 5. 0 g 与10 mL 甲醇混合并离心5 min, 将上层清液移入25 mL 容量瓶中, 下层油相再用甲醇提取 2 次, 提取液合并于25 mL 容量瓶中, 以甲醇定容此溶液也供 HPLC 分析用Di monsil C18 色 谱柱作固定相, 流动相为乙腈pH 6. 5 的磷酸盐缓冲溶液( 75+ 25) 混合液, 于245 nm 波长进行检 测测得在水基介质中的测定下限( 10S/ N ) 为0. 01 mg L- 1 , 在葵花籽油中的测定下限( 10S/ N ) 为0. 1 mg kg- 1 方法的回收率为89. 1%~ 97. 9%, 相对标准偏差( n= 9) 为2. 6%~ 7. 9% : 高 液相色谱法; 苯甲醛; 迁移量; 食品接触材料 : O652. 63 : A : 100 14020( 20 11) HPLC Determination of Benzaldehyde Migrated from Food Contacting Materials LI Ying, SUN iaoying, BAI Shuang, DAI Shouhai, LIU Li, LIANG Feng, CHEN uhui, LI Yongtao ( Shenz hen E ntr yEx it I nsp ect ion and Quar ant ine B ur ea u, Shenz hen 5 18045, Ch ina) Abstract: Amount of benz ldehyde migr ted from foodcont cting m teri ls ( e. g . , polystyrene) into 4 simul ted food mediums ( i. e. , 3 w terb sed mediums nd 1 f tb sed medium) w s determined by HPLC. In c se of w terb sed food, 3 portions of polystyrene s mple were reflux ed sep r tely in the 3 simul t ed mediums t 95 for 2 h, nd e ch of the ext r cts w s m de up to 100. 0 mL w ith the respective w terb sed food medium, nd used for H PLC n ly sis. In c se of f tb sed food, sunflow er seed oil w s t ken s simul ted food medium, nd the poly st yrene s mple w s so ked in the oil t 95 for 2 h in tightly stoppered conic l fl sk. Five gr ms of the cooled oil w ere t ken, mix ed w ith 10 mL of meth nol nd centrifuged for 5 min. The supern t nt w s tr nsferred to 25 mL volumetric fl sk. T he low er l yer w s