两个谷子CIPK基因在非生物逆境胁迫下的表达分析.pdfVIP

作物学报 ACTA AGRONOMICA SINICA 2016, 42(2): 295302 / ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ DOI: 10.3724/SP.J.1006.2016.00295 两个谷子CIPK 基因在非生物逆境胁迫下的表达分析 余爱丽1,2 赵晋锋1,2 王高鸿2 杜艳伟2 李颜方2 张 正2 郭二虎2 梁爱华1, 1 , 030006; 2 / , 046011 : CIPK /, 2 CIPK , SiCIPK6 SiCIPK16 SiCIPK6 1994 bp, 451 ; SiCIPK16 1885 bp, 473 , 2 2 CIPK PCR SiCIPK6 SiCIPK16 ABA, SiCIPK6 ABA , SiCIPK16 PCR SiCIPK6 SiCIPK16 , SiCIPK6 SiCIPK16 : ; CIPK ; Expression Analysis of Two CIPK genes under Abiotic Stress in Foxtail Millet 1,2 1,2 2 2 2 2 YU Ai-Li , ZHAO Jin-Feng , WANG Gao-Hong , DU Yan-Wei , LI Yan-Fang , ZHANG Zheng , GUO Er-Hu2 , and LIANG Ai-Hua1, 1Insitute of Biotechnology, Shanxi University, Taiyuan 030006, China; 2 Millet Research Institute, Shanxi Academy of Agricultural Sciences / Shanxi Key Laboratory of Genetic Resources and Breeding in Minor Crops, Changzhi 046011, China Abstract: CIPK (CBL interacting protein kinase) is a type of serine or threonine protein kinases, which plays an important role in response to stress. In this study, we identified two CIPK genes designated as SiCIPK6 and SiCIPK16 from foxtail millet (Setaria italica) genome using bioinformatics methods. The sequence analysis showed that SiCIPK6 has a length of 1994 bp in the genome, encoding 513 amino acids residues, and SiCIPK16 is 1885 bp, encoding 473 amino acids residues. These two genes have no al- t