细胞培养中珍贵贴壁细胞污染挽救方法的评价-首都医科大学学报.pdfVIP

2011 2 F eb. 20 11 32 1 Journal of C apitalM ed ical U niversity V o l. 32 N o. 1 [ do:i 10. 3969/ j. issn. 2011. 01. 026] 细胞培养中珍贵贴壁细胞污染挽救方法的评价 1, 2 1, 2* 1, 2 王 鸿 张 伟 孟 娜 1. ; 2. , ) , , , , laryngeal squam ou s carc inom a1, L SC1) , , DNA , 3 3 , 32 m g / L /, 3; /, 5~ 6 , 2 , , 8 , , , ; , , , 3 ; DNA , , 3, , , ; ; Q 813. 1+ 1 Evaluation ofM ethods for Res uing NovelAdherent Cell L ine Contam inated by Ba teria in Cell Culture 1, 2 1, 2* 1, 2 WANG H ong , ZHANG W ei , MENG N a 1. D ep artm ent of Otorhinolaryngo logy H ead and N eck Surgery, Beij ing Tongren H osp ita,l Cap italM edical Uni ersity; 2. K ey Laboratory of O torhino laryngology H ead and N eck Surg ery, B eij ing Institute of Otorhino laryngo logy ) AB STRACT Obje tive T o com pare the m ethods for rescuing the cell line contam inated by bacte ria. M ethods A ntib iotics or antib io tic com b ined w ith m acrophage or anmi a l inoculation w e re u sed to e lmi inate the bacte ria in laryng eal squamou s carcinom a cell line LSC1. T hen cy tokeratin CK AE 1/AE3) and DNA p lo idy in LSC1 ce lls w ere detec ted by mi munoh istochem ical and flow cy tom eter analysis. Results C efoperazone / Su lbactam at a dose of 32 m g / L w as the best one in three sensitiv e antib io tics and m ade LSC1 cells to surv ive three passages. Cefoperazone / Su lbactam com bined w ith m acrophage m ade L SC1 ce lls to surv ive 5~ 6 passages. But in the wt o m ethods, bacte ria appeared again w hen Cefopera zone / Su lbactam w as e lmi ina ted and the ce lls gradua lly d ied. LSC1 cells that exper ie