RNA干扰Livin基因对甲状腺乳头状癌的增殖及侵袭抑制作用及其分子机制.docVIP

RNA干扰Livin基因对甲状腺乳头状癌的增殖及侵袭抑制作用及其分子机制 　　【摘要】 目的：探?Livin对甲状腺乳头状癌TPC-1细胞增殖、凋亡及侵袭的影响以及可能的调控分子机制。方法：构建靶向Livin基因的慢病毒shRNA载体，转染甲状腺乳头状癌细胞株TPC-1，实验分为干扰组、阴性对照组和空白组。应用RT-PCR和Western印迹检测转染Livin-shRNA后细胞Livin mRNA和蛋白表达水平的变化。应用MTT、流式细胞仪、Western印迹与Transwell侵袭实验检测Livin对甲状腺乳头状癌细胞增殖、凋亡及侵袭的影响。并接种于裸鼠模型中，进一步确定Livin在体内对甲状腺乳头状癌细胞的影响。结果：慢病毒Livin-shRNA载体成功抑制TPC-1细胞中Livin mRNA和蛋白的表达；转染Livin-shRNA后TPC-1细胞的增殖能力受到明显抑制（P 　　【关键词】 甲状腺肿瘤； Livin； RNA干扰； 侵袭； 迁移； 动物模型 　　Inhibition on Proliferation and Invasion of Human Thyroid Papillary Cancer Cells by RNA Interference Livin Gene Expression and Its Possible Molecular Mechanism/XUE Dong，JIN Ji-hai，XIA Xiu-liang，et al.//Medical Innovation of China，2017，14（14）：004-008 　　【Abstract】 Objective：To investigate the effect of Livin on the proliferation，apoptosis and invasion of human thyroid papillary cancer cell line TPC-1，and its possible molecular mechanism.Method：Livin was silenced by using RNA interference.In this experiment，three groups were designed：the experimental group，the negative control group and the blank control group.The expression of Livin at mRNA and protein levels were detected by reverse transcription PCR and Western blot respectively.MTT，FCM，transwell and Western blot were utilized to analyze the result and specific molecular mechanism of Livin on TPC-1 cancer cells by transfected.The expressions of AKT，p-AKT，PDK1 and PTEN in TPC-1 cells were detected after transfection.Nude mice models inoculated TPC-1 cells were used to further clarify the effect of Livin on thyroid papillary cancer cells in vivo.Result：The stable Livin silencing cells line was successfully established.The expression of Livin at mRNA and protein levels was reduced significantly（P 　　【Key words】 Thyroid neoplasm； Livin； RNA interference； Invasion； Migration； Animal model 　　First-author’s address：The People’s Hospital of Binzhou，Binzhou 256610，China 　　doi：10.3969/j.issn.1674-4985.2017.14.002 　　甲状腺乳头状癌的发病率正逐年上升。Livin与肿瘤的发生、恶性侵袭和转移具有密切相关性[1-2]。笔者前期研究结果已发现Livin在甲状腺乳头状癌组