不同添加剂对聚乙烯醇固定化锰氧化细菌的影响-北京工业大学学报.PDF

第39卷 第11期 北 京 工 业 大 学 学 报 Vol.39 No.11 2013年 11月 JOURNAL OF BEIJING UNIVERSITY OFTECHNOLOGY Nov. 2013 不同添加剂对聚乙烯醇固定化锰氧化细菌的影响 张长利,杨摇 宏,王景晶,梅摇 青 (北京工业大学 北京市水质科学与水环境恢复重点实验室,北京摇 100124) 摘摇 要:为了研究海藻酸钠、琼脂、碳酸钙、活性炭4种添加剂对聚乙烯醇固定化锰氧化细菌的影响,通过环境扫描 电镜进行观察. 结果表明:不加添加剂的聚乙烯醇交联后内部分布不均匀,结构中存在外致密层;加入琼脂可明显 缓解外致密层的形成,微观孔隙数量增加;加入碳酸钙后聚乙烯醇颗粒直径缩小,内部孔隙的壁厚较大;碳酸钙和 活性炭的加入不利于聚乙烯醇形成微观孔隙;海藻酸钠和琼脂恶化了聚乙烯醇颗粒的水溶膨胀性,而碳酸钙和活 性炭有助于改善水溶膨胀性;当添加剂加入量低于1%时,4 种添加剂都可改善聚乙烯醇的机械强度,加入过多添 加剂会使机械强度下降;加入活性炭有利于提高聚乙烯醇固定锰氧化细菌的活性,而碳酸钙使固定细菌的机械强 度改善最为明显. 关键词:聚乙烯醇;细胞固定;锰氧化细菌;添加剂 中图分类号:X52 文献标志码:A 文章编号:0254-0037(2013)11-1710-06 Effect of the Co鄄composite Matters on the Immobilized Manganese鄄oxidizing Bacteria by PVA ZHANG Chang鄄li,YANG Hong,WANGJing鄄jing,MEI Qing (Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology,Beijing 100124,China) Abstract:Four kinds of co鄄composite matters including alginate,agar,calcium carbonate and activated carbon were mixedwithpolyvinyl alcohol (PVA)for immobilizingthemanganese鄄oxidizingbacteria. The internal structureofthePVAgelbeadswastestedandanalyzedby scanningelectronmicroscope. Adense layer exists in the surface of PVA gel beads with an unequal distribution,and adding agar is useful for overcoming this shortcoming and increasing the number of internal pore. Adding calcium carbonate decreases the diameter of thegelbeadsandimprovesthethicknessof theinternalporewall. The solution and expansion of PVA beads may be worsen after adding sodium alginate and agar and better with activated carbon and calcium carbonate. All the four kinds of co鄄composite matters are useful fo